ANILIN COLOURS GIVING INDIRECT NUCLEAR STAINS. 55 



CHAPTEE VIII. 



ANILIN* COLOURS GIVING INDIRECT NUCLEAR STAINS 

 (FLEMMING'S METHOD). 



95. Very few anilins give a precise nuclear stain by the 

 direct method ( 92) . Two of them methyl green and Bis- 

 marck brown are pre-eminently nuclear stains. Many of 

 the others for instance, safranin, gentian, and especially 

 dahlia, may be made to give a nuclear stain with fresh tissues 

 by combining them with acetic acid ; but in ninety-nine cases 

 out of a hundred are not so suitable for this kind of work as 

 the two colours first named, which practically form a class 

 apart. 



Again, very few anilins give a pure plasmatic stain (one 

 leaving nuclei unaffected) . The majority give a diffuse stain, 

 which in some few cases becomes by the application of the 

 decolouration or indirect method (92) the most precise and 

 splendid stain as yet obtainable by any means. 



The indirect staining method, or Flemming's method, will 

 form the subject of the present chapter, and the remaining 

 anilins will be treated of in the next chapter. 



The following list shows the colours treated of in the two 

 chapters. 



In Chapter VIII. 



COLOUES GIVING INDIRECT NUCLEAR STAINS (FlEMMING's 

 METHOD). 



(The order in this chapter is one dictated merely by convenience 



of exposition.) 

 Red. 



Safranin . . . 103 Fuchsin (Rosein, Rubin, 

 Magdala red (Naphtha- Magenta, Solferino, 



linred) , . .104 Corallin) . . .104 



* The word " anilin " is here used in the popular sense, to include all 

 coal-tar colours. 



