66 ANILIN COLOURS GIVING INDIRECT NUCLEAR STAINS. 



stains by the indirect process. FLEMMING (Arch. f. mik. Anat., xix, 1881, 

 pp. 317 and 742) mentions the following : 



MAGDALA RED (NAPHTHALIN RED, ROSE DE NAPHTHALINE). Nearly if 

 not quite as good a stain as any of the foregoing, and superior to all except 

 safranin in respect of permanency. This and the following should, as far 

 as is yet made out, be used in alcoholic solution diluted with about one half 

 of water, and be washed out with pure alcohol, followed by clove oil. 



Mauvein and Rouge Fluorescent are good stains, but colour some 

 nuclei more deeply than others in the same preparation. 



Solid Green (perhaps the same as the analin green discussed above) is 

 very elective for nucleoli. 



Puchsin (meaning the basic fuchsins, a series of Rosanilin salts having 

 very similar reactions and found in commerce under the names of FUCHSIN, 

 ANILIN RED, RUBIN, ROSEIN, MAGENTA, SOLFEEINO, COEALLIN). A good 

 but somewhat weak stain, by the alcohol method. Good results are obtained 

 by substitution in the following manner (GEASEE, Deutsche Zeit.f. Chir- 

 urgie, xxvii, 1888, pp. 538584; Zeit.f. wiss. Mik., v, 3, 1888, p. 378). 

 You either employ the colour as directed for methyl violet (post, 107), or 

 you stain for twelve to twenty -four hours in a dilute aqueous solution, wash 

 out for a short time in alcohol, stain for a few minutes in aqueous solution 

 of inethylen blue, and dehydrate with alcohol. A double stain. Chromatin 

 and nucleoli red, all the rest blue. 



OEANGE, precise but weak. 



BISMAECZ BEOWN is not very satisfactory with chromic objects. With 

 alcohol objects it gives a good chromatin stain, but cannot be thoroughly 

 removed from cytoplasm by any means yet discovered. 



To these may be added 



METHYL VIOLET, perhaps best used according to the method of Resegotti 

 given in the last ; and (according to GEIESBACH, Arch.f. mik. Anat., xxii, 

 p. 132). 



TEOPJEOLIN 000, No. 2 (OEANGE n ; CHEYSAUEEIN, j8 NAPHTOLOEANGE), 

 a fine dark orange stain, and 



ROCELLIN (ECHTEOTH, OE8EILLIN No. 3, RlJBIDIN, LA RAUVAEIENNK), a 



cherry-red stain. 



BENZOAZUEIN has been lately recommended by MAETIN (see Zeit.f. wiss. 

 Mik. t vi, 2, 1889, p. 193). Stain for an hour or so in dilute aqueous solu- 

 tion and wash out with HC1 alcohol. 



