RANVIER'S HJIMATOXYLIN. 95 







toxylin." In 1885 matters were set right by Prndden's explaining that the 

 stain was the invention of Delafield, and publishing the correct formula here 

 quoted (in the formula as published by Flemming, the proportions are some- 

 what different). It is within the bounds of possibility that after a generation 

 this formula may no longer be quoted as Grenadier's ; but up to the present 

 date the Conservatives remain nearly as numerous as the Reformers). 



To 400 c.c. of saturated solution of ammonia-alum add 4 gr. 

 of hsematox. crist. dissolved in 25 c.c. of strong alcohol. 

 Leave it exposed to the light and air, in an unstoppered 

 bottle, for three or four days. Filter, and add 100 c.c. of 

 glycerin and 100 c.c. of methylic alcohol (CH 4 0). Allow the 

 solution to stand until the colour is sufficiently dark, then filter 

 and keep in a tightly stoppered bottle. 



This solution keeps well it may be said to keep for years. 

 .It is well to allow it to ripen for at least two months before 

 .using it. 



This famous solution is certainly one of the best hasmatoxylih 

 .stains yet published. It is extremely powerful, and when 

 properly used, very precise. For staining, enough of the 

 .solution should be added to pure water to make a very dilute 

 stain ; and even then care should be taken not to leave objects 

 too long in the fluid. 



175. Bohmer's Haematoxylin (Arch.f. mik. Anat., iv, 1868, p. 

 345; Aerzt. IntelligenzbL, Baiern, 1865, No. 38). Make (A) a 

 solution of pure haematoxylin (9j) in absolute alcohol (Jss), and 

 (B) a solution of aluinen depuratum (gr. ij) in water (j), (or, 

 (A), haematox. crist. 1 part, alcohol 12 parts, and (B), alum 1 

 part, water 320) . For staining, add two or three drops of A 

 to a watch- glassful of B. 



The staining solution ought not to be made up at the moment 

 of using, but should be made up beforehand and allowed to 

 ripen for some days. The alcoholic solution of haematoxylin 

 may be kept in stock ; it becomes brown, but does not lose its 

 properties. 



Washing out may be done with a 0'5 per cent, solution of 

 alum in water. 



This formula gives a very fine stain, but is not so certain in 

 its results as Delafield's. 



176. Ranvier's Hesmatoxylin (Comptes rend. Ac. Sc., 1882, 2 sem., 

 t. 95, p. 1375). If solutions prepared according to the formula of Bohmer be 

 kept for some weeks they will be found to furnish an abundant precipitate. 



