124 COMBINATION STAINS. 



soluble in alcohol to ten grammes of absolute alcohol, for 

 sections of nerve-centres). Clear with turpentine, without 

 further treatment with alcohol, and mount in balsam. 



The sections should appear of a fine dark violet when taken 

 from the anilin : they are extremely transparent under the 

 microscope. Nerve-cells and axis-cylinders, reddish violet; 

 blood-vessels, bluish violet, and so sharply marked out that 

 the preparations have the aspect of injections. The connective 

 elements are stained of a nearly pure blue, so -that it is easy 

 to distinguish them from the nervous elements. 



Applicable to all kinds of tissues, but especially to sections 

 of nerve-centres. 



Recent authors recommend instead of anilin blue, Bleu de 

 Lyon, dissolved in 70 per cent, alcohol acidulated with 

 acetic acid (MAURICE and SCHULGIN), or Bleu lumiere, which 

 has hardly any effect on nuclei. 



The solutions of both these colours should be extremely 

 dilute. They may be used for staining in the mass. 



232. Picro-Carmine and Iodine Green (STIRLING, Journ.Anat. 

 and PhysioL, xv, 1881, p. 349, et seq.). Stain picro-carmine, 

 wash in acidulated water (acetic acid), stain iodine green. 

 Iodine green stains very rapidly, and care must be taken not 

 to over-stain. Rinse in water, dehydrate rapidly, clear with 

 clove oil, mount in dammar. (All preparations stained with 

 iodine green must be mounted in dammar.) 



Iodine green has a specific action on adenoid tissue and 

 mucous glands, which it stains of a bright green. 



233. Picro-Carmine and Methyl Green (MAX FLESCH, Zool.Anz., 123, 

 1882, p. 554). Sections of cartilage, skin, and glands made from tissues 

 hardened in Muller's solution and alcohol, were stained with picro-carmine, 

 and subsequently (not " previously," as erroneously stated in Journ. Roy. 

 Mic. Soc. (N.S.), ii, 1882, p. 883) with an aqueous solution of commercial 

 methyl green made of such a strength that the sections are just distinguish- 

 able in a watch-glassful of the solution when placed on a light ground. 



The method is easy, gives good differentiations, but the stain does not 

 appear likely to be permanent. Mount in balsam. 



234. Picro-Carmine, Rosein, and Anilin Blue; or Picro-Carmine, 

 Anilin Violet, and Anilin Blue ; or Picro-Carmine, Anilin Violet, 

 and Iodine Green; or Picro-Carmine, Rosein, and Iodine Green 

 (HENEAGE GIBBES, Jown. Roy. Mic. Soc., iii, 1880, p. 392). Make a dilute 

 solution of picro-carmine (about 10 drops to a watch-glass of water), stain in 

 it for about half an hour, wash out for an hour in water acidulated with a 



