THE ANILIN DOUBLK STAINS. 127 



There is no doubt as to this being a very fine and usefuir 

 stain. 



See also Comptes Rendus, 1879, p. 1039 (Ire ser.), and Arch, 

 de PhysioL, 1881, p. 640. 



240 Hsematoxylin and Benzo-purpurm (ZSCHOKKE). For this 

 and the combination with Delta Purpurin, see ante, 114. 



241. Haematoxylin and Iodine Green. (STIELING, Journ. of Anat. 

 and PhysioL, xv, 1881, p. 353). Stain not too deeply with logwood, and 

 then stain with iodine green. (Mucus-glands of tongue green, serous glands 

 haematoxylin). 



242. HaBmatoxylin and Nitrate of Bosanilin (Lisx, Zeit. f. wiss. 

 Mik., ii, 1885, p. 149). Stain for twenty-four hours in the dilute haematoxylic 

 glycerin of which the formula was given just now, 238, wash, and stain 

 for about ten minutes in solution of nitrate of rosanilin, then rinse with 

 water, dehydrate with absolute alcohol, and clear. Recommended especially 

 for mucus-glands, epithelia, and cartilage. 



243. Hsematoxylin and Safranin. This celebrated combina- 

 tion, which was used to such good effect by RABL in his 

 classical researches on nuclei (Morph. Jahrb., x, 1884, p. 215), 

 does not strictly belong to this subdivision, the safranin 

 being the primary stain, though used after the haematoxylin. 

 You stain very lightly with haematoxylin, so lightly that the 

 stain would not be of any use by itself (Rabl uses very dilute 

 Delafield's solution, for twenty-four hours) ; wash out first with 

 water, and then with alcohol acidulated with HCL, then 

 stain for some hours in (Pfitzner's) safranin, and wash out 

 with pure alcohol. Rabl certainly was not far wrong when 

 he wrote " This method is unequalled by any other " For 

 richness of detail in both nucleus and cytoplasm and tissue 

 this method has indeed hardly been equalled. 



244. Hsematoxylin and Metallic Stains. The conditions 

 under which haematoxylin can usefully be employed for 

 staining impregnated tissues have been discussed under the 

 heads of Gold and Silver. It only remains here to remind 

 the reader that haematoxylin works very well after osmic acid. 



Other Combinations. 



245. The Anilin Double-Stains. These very important com- 

 binations are so numerous that only a small proportion of 

 them can be mentioned here. 



