METHYL GREEN AND EOSIN. 129 



driven out of the tissues by the methylen blue, a result which 

 is not attained by washing with alcohol alone, either pure or 

 acidified. 



247. Baumgarten's Triple Stain (Bull Soc. Belg. Mic., 7, 1887, and 

 14, 1888, p. 146 ; Journ. de Microgr., 1888, p. 415 ; Journ. Roy. Mic. Soc., 

 1887, p. 676, and 1889, p. 149). Consists of borax-picro-carmine ( 163), 

 picric acid, gentian violet, iodine, and acidulated alcohol and picric acid in 

 succession. Very complicated, and too delicate to manipulate for ordinary 

 work. 



248. Garbini's Safranin and Anilin Blue (Zool. Anz., 1886, p. 26 ; 

 and Zeit. f. wiss. Mik., v, 2, 1888, p. 170). Stain (sections) one to four 

 minutes in anilin blue (1 per cent, in water, with 1 per cent, of absolute 

 alcohol). Wash in 1 per cent, solution of carbonate of lithium until the colour 

 has nearly disappeared. Treat for five to ten minutes (until the colour has 

 come back) with 0'5 per cent, hydrochloric acid. Distilled water. Safranin 

 (0'5 gr. in 150 c.c. one-third alcohol) ten to twenty minutes, heating for a 

 minute or two. Dehydrate with methyl alcohol (CH 3 ), finish the differentia- 

 tion of the stain in a mixture of one part oil of cedar with two parts clove 

 oil, and pass through xylol into balsam. 



Somewhat complicated, but a sharp stain, rich in detail, and valuable in 

 so far as there are not many blues that give very satisfactory results with 

 safranin. 



You may dehydrate and clear in the ordinary way with alcohol and clove 

 oil if you like, and, as far as I can see, without inconvenience. 



249. Safranin and Indigo-Carmine (KOSSINSKI, Zeit. /. wiss. 

 Mik.j vi, 1, 1889, p. Gl). Stain sections ten to twenty minutes 

 in saturated aqueous solution of indigo-carmine, wash with 

 water and with alcohol, and stain with safranin (0*5 per cent, 

 in dilute alcohol), dehydrate and mount. 



Safranin and Nigrosin is a combination also recommended by 

 the same author. Stain for three to five minutes, in 0*1 per 

 cent, aqueous solution of nigrosin, and proceed as before. 



250. Methyl Green and Eosin (CALBERLA, Morph. Jahrb., iii, 

 1877, 3 Hft., p. 625). Mix 1 part of eosin with 60 parts of 

 methyl green, and dissolve the mixture in warm 30 per cent, 

 alcohol. 



Sections stain in this solution in five or ten minutes ; they 

 should be quickly washed in successive alcohols, and mounted 

 in balsam or glycerin. 



251. Methyl Green and Eosin (LiST, Zeit. f. wiss. Mik., ii, 

 1885, p. 147). Stain for a few minutes in a mixture of three 







