ROSE BENGALE AND IQDlNE GREEN. 131 



to fifteen minutes in O'OOOl per cent, aqueous solution of~ 

 nitrate of rosanilin, wash out (rapidly) with absolute alcohol, 

 and clear. 



255. Anilin Green and Bismarck Brown (LIST, 1. c.). To be 

 used in the same way as methyl green and Bismarck brown 

 supra, ( 253), and giving much the same results. 



256. Anilin Green and Eosin (SCHIEFFERDECKER, Arch f. mik. 

 Anat., xv, 1878, p. 30). Stain in a solution made by adding 

 a few drops of aqueous solution of eosin to a watch-glassful of 

 alcohol, for from half an hour to several hours, wash with water 

 and stain for a few minutes in 1 per cent, aqueous solution of 

 anilin green. Wash with water, wash out with alcohol and 

 clear with clove oil. 



This combination possesses a rich selectivity, especially for 

 connective and glandular tissues. 



LIST (1. c., 251) recommends this combination, which he 

 employs in the following manner : Stain for a quarter of an 

 hour in the eosin solution there quoted, rinse with alcohol, and 

 stain for a quarter of an hour in 0'5 per cent, aqueous solution 

 of anilin green diluted with 3 volumes of absolute alcohol. 

 Wash out with absolute alcohol and (as soon as the colour of 

 of the eosin begins to reappear) clear with bergamot oil or 

 xylol. 



257. Dahlia and Eosin (SCHIEFFERDECKER, I. c.). Proceed as 

 for anilin green and eosin ( 256), using a 1 per cent, aqueous 

 solution of dahlia. 



258. Methyl Violet and Eosin (SCHIEFFERDECKER, Z. c.). Pro- 

 ceed as before, using a 1 per cent, aqueous solution of methyl 

 violet. 



269. Iodine Green and Eosin (STIRLING, Journ. Anat. and PhysioL, 

 xv, 1881, p. 354). Stain in alcoholic solution of eosin, wash in acidulated 

 water (1 per cent, acetic or hydrochloric acid), stain with iodine green. 



260. Eose Bengale and Iodine Green (GRIESBACH, Zool Anzeig., 135, 

 1883, p. 172). The method consists in staining very quickly in a strong 

 aqueous solution of rose bengale (the section must have been soaked in 

 water before bringing into the stain), washing out with water, and staining 

 for a few seconds in iodine green. The sections may then be mounted, or 

 may be further treated with bleu de Lyon. This is done by treating them 

 for five minutes with absolute alcohol, and staining for two or three seconds 



