132 COMBINATION STAINS. 



in a solution of bleu de Lyon in 40 per cent, alcohol. The sections appear 

 not to take the blue stain, but it becomes visible as soon as they are mounted. 

 They are to be dehydrated in absolute alcohol, cleared in oil of anise-seed of 

 0*99 sp. gr., and 1'811 refractive index, and mounted in dammar. The method 

 gives in some cases very striking differentiations, but the results are by no 

 means constant, and do not admit of being generalised. 



261. Rosem and Anilin Blue, Kosein and Anilin Green, Anilin 

 Violet and Anilin Blue, Anilin Violet and Anilin Green (see H. 

 GIBBES, Journ. Roy. Mic. Soc., 1880, p. 391). 



262. Metallic Stains and Anilin Colours. Most of the coal- 

 tar colours above quoted may be employed after a metallic 

 impregnation. The combination of safranin with gold chloride, 

 first recommended by Pfitzner, is a classical stain. Eosin 

 may usefully be made to follow nitrate of silver impregnation. 

 And other combinations may be found useful on occasion. 



