CHLORIDE AND ACETATE OF COPPER. 199 



4TH FLUID. " Marine animals require a stronger fluid of this 

 kind, made by adding about 2 ounces more salt to the last. 



367. Owen's Fluid (quoted from VOGT et YUNG, Traite 

 d'Anat. comp. pratique, p. 19). 



Corrosive sublimate . . '014 -grammes. 

 Alum . . . . . ' 79 ,, 



Salt 137 



Water . . 1680 



Said to be very useful for the preservation of soft-bodied 

 animals. 



368. Gilson's Fluid (CARNOY'S Biologie Cellulaire, p. 94). 

 Alcohol of 60 per cent. . . .60 c.c. 



Water 30 



Glycerin . . . . . 30 



Acetic acid (15 parts of the glacial to 



85 of water) . . . . 2 



Bichloride . . . . .0*15 grammes. 

 A really excellent medium for the study of fine cellular 

 detail with well-fixed objects. 



369. Gage's Albumen Fluid (Zeit.f. wiss. Mik., 1886, p. 223). 

 White of egg . . . . .15 c.c. 



Water 200 



Corrosive sublimate . . . .0*5 grammes. 

 Salt . . ... 4 



Mix, agitate, filter, and preserve in a cool place. Re- 

 commended for the study of red blood-corpuscles and ciliated 

 cells. 



Other Fluids. 



370. Chloride and Acetate of Copper (RIPAET et PETIT'S fluid, 

 Brebissonia, 1880, p. 92 ; CARNOY'S Biol Cell., p. 95). 



Camphor water (not saturated) . 75 grammes. 



Distilled water. .... 75 

 Crystallised acetic acid . . 1 



Acetate of copper .... 0*30 

 Chloride of copper .... '0'30 

 This is certainly a most valuable medium for work with 

 delicate fresh tissues. It may be used in combination with 

 methyl green, which it does not precipitate. The most 



