BANVTER'S CARMINE GELATIN MASS. 219 



Sulphide of sodium (sol. sat.) . . 30 c.c. 



Glycerin 50 



Mix with agitation and combine with 3 vols. of vehicle. 



438. Scheele's Green Colouring Mass (Ibid., p. 37). 

 Take 



Arseniate of potash (saturated solution) 80 c.c. 



Glycerin . .'."', . . 50 



and 



Sulphate of copper (saturated solution). 40 



Glycerin 50 



Mix and combine with 3 vols. of vehicle. 



438 a. Anilin Colouring Masses (Ibid. t p. 37). The anilin 

 colours have, for injections, the great fault of being soluble 

 in alcohol ; fuchsin is soluble in water, in alcohol, and in 

 glycerin ; it therefore cannot be employed with a gelatin or 

 glycerin vehicle. Anilin blue, violet, yellow may be combined 

 with these vehicles after dissolving in a small quantity of 

 alcohol; and (alcohol being avoided for hardening purposes) 

 the injected organs may be preserved in glycerin. 



Carmine Gelatin Masses. 



439, Ranvier's Carmine Gelatin Mass (Traite technique, p. 

 116). Take 5 grammes Paris gelatin, soak it in water for 

 half an hour, or until quite swollen and soft ; wash it ; drain 

 it ; put it into a test-tube and melt it, in the water it has ab- 

 sorbed, over a water-bath. When melted add slowly, and 

 with continual agitation, a solution of carmine in ammonia, 

 prepared as follows : 2 grammes of carmine are rubbed-up 

 with a little water, and just enough ammonia, added drop by 

 drop, to dissolve the carmine into a transparent solution. 



When the carmine has been added to the gelatin you will 

 have about 15 c.c. of ammoniacal solution of carmine in 

 gelatin, if the operations have been properly performed. This 

 solution is to be kept warm on the water-bath, whilst you 

 proceed to neutralise it by adding cautiously, drop by drop, 

 with continual agitation, a solution of 1 part of glacial acetic 

 acid in 2 parts of water. (When the mass is near neutrality, 

 dilute the acetic acid still furthur.) The instant of saturation 

 is determined by the smell of the solution, which gradually 



