GHEOMIC ACID. 243 



Potassium chloride .... 0*4 



Sodium chloride ..... 0*3 



Phosphate of soda ..... 0*2 



Calcium chloride . . . . . 0*2 



1-1 



This is dissolved in 100 parts of water, saturated with 

 carbonic acid, and the solution combined with water and 

 solutio Miilleri, one volume of the solution being combined 

 with half a volume of Miiller's solution and a volume of 

 water. 



In either case the Miiller's solution may be replaced by a 

 2J per cent, solution of chromate of ammonia. The best 

 results were obtained when the solutions were saturated with 

 the C0 2 just before using. 



The tissues are isolated by teasing and shaking, and speci- 

 mens mounted in concentrated acetate of potash. 



505. LANDOIS'S Solution (Arch. f. mik. Anat., 1885, p. 445). 

 Saturated sol. of neutral chromate of ammonia 5 parts. 

 Saturated sol. of phosphate of potash . 5 

 Saturated sol. of sulphate of soda . . . 5 

 Distilled water. 100 



To be used in the same way as chromic acid : small pieces 

 of tissue are macerated for one to three, or even four or five 

 days, in the liquid, then brought for twenty-four hours into 

 ammonia carmine diluted with one volume of the macerating 

 liquid. 



GIERKE particularly recommends this liquid for all sorts of 

 macerations, but especially for the central nervous system, for 

 which he finds it superior to all other agents. It is also re- 

 commended for the same purpose by NANSEN (v. Zeit. f. wiss. 

 Mik., v, 2, 1888, p. 242). 



506. Permanganate of Potash. Has an action similar to that 

 of osmic acid, but more energetic. Is recommended, either 

 alone or combined with alum, as the best dissociating agent 

 for the fibres of the cornea (ROLLETT, Strieker's Handbuch, 

 p. 1108). 



507. Chromic Acid. Generally employed of a strength of 

 about 0'02 per cent. Specially useful for nerve tissues and 



