246 MACERATION AND DIGESTION. 



cerin, 1 part of methyl alcohol, and 20 parts of distilled water. 

 Macerate for several days (perfectly fresh tissue). 



Digestion. 



521. BEALE'S Digestion Fluid (Archives of Medicine, i, 1858, 

 pp. 296 316). The mucus expressed from the stomach 

 glands of the pig is rapidly dried on glass plates, powdered, 

 and kept in stoppered bottles. It retains its properties for 

 years. Eight-tenths of a grain will dissolve 100 grains of 

 coagulated white of egg. 



To prepare the digestion fluid, the powder is dissolved in 

 distilled water, and the solution filtered. It filters readily. 

 Or the powder may be dissolved in glycerin. The tissues to 

 be digested may be kept for some hours in the liquid at a 

 temperature of 100 F. (37 C.). 



522. BKUCKE'S Digestion Fluid (from CARNOY'S Biologie 

 Gellulaire, p. 94). 



Glycerinated extract of pig's stomach . . 1 vol. 

 0'2 per cent, solution of HC1 . . .3 vols. 

 Thymol, a few crystals. 



523. BICKFALVI'S Digestion Fluid (Centralbl. f. d. med. Wiss., 

 1883, p. 833). One gramme of dried stomachal mucosa is 

 mixed with 20 c.c. of 0*5 per cent, hydrochloric acid, and put 

 into an incubator for three or four hours, then filtered. 

 Macerate the tissue in the solution for not more than half an 

 hour to an hour. 



524. KUSKOW'S Digestion Fluid (Arch. f. mik. Anal., xxx, 

 p. 32, cf. Zeit.f. wiss. Mik., iv, 3, 1887, p. 384). One part of 

 pepsin dissolved in 200 parts of 3 per cent, solution of oxalic 

 acid. The solution should be freshly prepared, and the 

 objects (sections of hardened Ligamentum Nuchae) remain in 

 it at the ordinary temperature for ten to forty minutes. 



525. SCHIEFFERDECKER'S Pancreatin Digestion Fluid (Zeit. f. 

 wiss. Mik., iii, 4, 1886, p. 483). Solution of pancreatin in 

 water. Schiefferdecker employs the " Pankreatinum siccum " 

 prepared by Dr. Witte, Kostock. A saturated solution is 

 made in distilled water, cold, and filtered. Pieces of tissue 

 (epidermis) are macerated in it for three to four hours at 



