KUHNE'S METHODS. 247 



about body temperature. Nuclei are preserved, and the_ 

 forms of prickle-cells well shown. 



526. KUHNE'S Methods (see Unters. a. d. Phys. Inst. Univ. 

 Heidelberg, i, 2, 1877, p. 219). Trypsin is prepared by ex- 

 tracting ox-pancreas with alcohol and ether, and evaporating 

 to dryness. One part of the product is heated for three to 

 four hours at a temperature of 40 C. with five to ten parts of 

 O'l per cent, solution of salicylic acid, and the solution forced 

 through linen and filtered cold. 



