288 CYTOLOGICAL METHODS. 



Arch. f. mik. Anat., xx, 1881, p. 3). The ova are stained on 

 the slide by adding the stain at the edge of the cover. Saf ranin 

 or other nitro- or anilin-colours may be used. As soon as 

 the entire ovum is of a dark colour the stain is drawn off 

 with blotting-paper, and acetic acid of 1 per cent, added. 

 Schneider's acetic carmine (Zool. Anzeig., 1880) (see 154) is 

 very convenient, and gives good results. (For the details of 

 the manipulation by which these reagents are added and drawn 

 off on the slide it is well to consult the article quoted, p. 6.) 



Another good method is as follows : Segmenting ova are 

 treated with a mixture of 40 to 50 parts of concentrated 

 nitric acid with 60 to 50 parts water. Wash with water until 

 all the yellow stain of the nitric acid has disappeared ; stain 

 with Schneider's acetic carmine, and mount in glycerin. (The 

 preparations cannot be said to be permanent, as after a time 

 the stain darkens in such a way as to render the nuclear 

 figures unrecognisable.) 



610. Other General Cytological Methods. 

 TIZZONI (Bull delle Sci. Med. di Bologna, 1884, p. 259). 

 BAUMGAETEN (Zeit.f. wiss. Mik., i, 1884, p. 415). 

 BIZZOZEEO (ibid., iii, 1886, p. 24). 



BABES (ibid., iv., 4, 1887, p. 470). 



ZWAAEDEMAKER (ibid., iv, 2, 1887, p. 212). 



NISSEN (Arch. f. mik. Anat., 1886, p. 338) stains material fixed in 

 Flemming and sectioned, by the method of GRAM (see above, 102). 



SCHOTTLANDER (Arcli. f. mik. Anat., xxxi, 1888, p. 426 ; Zeit. f. wiss. 

 Mik., v, 4, 1888, p. 515 (cell-division in the corneal endothelium). 



611. Cytology of the Ovum (Ascaris). 



VAN BENEDEN (Arch, de Biol., iv, 1883, p. 279). 



CARNOY (La Cellule, ii, 1, 1886, pp. 17, 18 ; iii, 1, 1886, p. 6 ; iii, 2, 1887). 



VAN BENEDEN et NEYT (Nouv. Rech. sur la Fee., &c., separate, 1887, or 

 Bull. Acad. roy. d. Sc. de Bruvelles, 1887, iii ser., xiv ; also Journ. Roy. 

 Mic. Soc., 1888, p. 508). 



BOVERI (Zellen-Studien, in Jen. Zeit. f. Naturw., xxi, 1887, p. 423, or 

 separate ; also Journ. Roy. Mic. Soc., 1888, p. 664). 



ZACHARIAS (Anat. Anz., iii, 1888, p. 24 ; also Journ. Roy. Mic. Soc., 

 1888, p. 663). 



VAN GEHUCHTEN (ibid., p. 237). 



KULTSCHITZKY (Arch, f. mik. Anat., xxxi, 1888, p. 567) recommends for 

 fixing ;i mixture of three parts of acetic ether with one part of absolute 

 alcohol. Clears for mounting in concentrated acetic acid, and mounts in a 

 mixture of acetic acid and balsam. An excellent resume of the technical 

 part of these papers is to be found in Zeit.f. wiss. Mik., v, 3, 1888, p. :{(!7. 



