298 MUSCLE AND TENDON. 



CHAPTER XXVIII. 



MUSCLE AND TENDON (NERVE-ENDINGS). 



Striated Muscle. 



629. Sections (ROLLETT, Denkschr. math, naturw. Kl. k. Acad. 

 Wiss., Wien, 1885; Zeit.f. luiss. Mik., 1886, p. 92). Besides 

 the usual section methods, the following methods of Rollett 

 should be noted: (1) The method mentioned above, 312, 

 of freezing living tissue in white of egg. (2) The same 

 method applied to recently fixed muscle. (3) For hardened 

 muscle, Rollett prefers celloidin (soak for twenty-four or forty- 

 eight hours in a very thin celloidin solution, then for twenty- 

 four hours in a solution of one part of celloidin in four parts 

 of a mixture of equal parts of alcohol and ether, then gradual 

 evaporation of the mass in a test tube to a gelatinous consis- 

 tency, followed by hardening for twenty-four hours in a mix- 

 ture of two parts of 93 per cent, alcohol with one of water) . 

 Rollett stains for several hours in Renaut's haomatoxylic 

 glycerin diluted with water to a very light violet tint. Dehy- 

 drate with alcohol, clear with origanum oil, and mount in 

 dammar. 



630. Dissociation. See Chapter XXIII. 



LANGEBHANS* methods for Amphioxus (Arch.f. mik. Anat., 1875, p. 291). 

 For isolation of the muscle-plates macerate the fresh animal in 20 per 

 cent, nitric acid. 



For isolation of the nervous system macerate an animal for three days in 

 20 per cent, nitric acid, then place it for twenty-four hours in water, and 

 shake forcibly. The whole of the nervous system may thus be separated, 

 almost down to the finest peripheral terminations of nerves. 



631. Motor Plates (FISCHER, Arch.f. mik. Anat. } 1876, p. 365) . 

 In these researches Fischer used for mammals the gold- 

 method proposed by LOWIT (Wien. Sitzgsber., Bd. Ixxi, Abth. 



