302 MUSCLE AND TENDON. 



This process colours terminal arborescences. After-blackening is said to 

 be almost entirely absent. 



Tendon. 



635. Corpuscles of Golgi (RANVIER, Traite, p. 929). Take 

 the tendon of the anterior and superior insertion of the gemini 

 muscles of the rabbit. Free it as far as possible from adherent 

 muscle-fibres. Treat it according to the formic-acid and gold 

 method ( 208), and after reduction of the gold scrape the 

 tendon with a fine scalpel in order to remove the muscle-fibres 

 that mask the "musculo-tendinous organs. " 



636. Corpuscles of Golgi (in the tendous of the motores 

 bulbi oculi) (V. MARCHI'S methods, Archivio per le Scienze 

 Mediche, vol. v, No. 15). The enucleated eyes, together with 

 their muscles, were put for not less than three days into 

 2 per cent, bichromate of potash. The muscles and tendons 

 were then carefully dissected out, stained with gold chloride 

 and osmic acid (Golgi's method, supra, 633) and by the 

 following methods suggested by MANFREDI. 



(1). The muscles and tendons removed from the bichromate 

 solution are put for half an hour into solution of arsenic acid 

 or into a 1 per cent, solution of acetic acid. They are then 

 passed directly into 1 per cent, gold chloride, half an hour; 

 distilled water ; then reduced in sunlight (until a deep violet 

 colour is obtained) in 1 per cent, arsenic acid solution, which 

 is changed as fast as it becomes brown. 



(2). The muscles and tendons removed from the bichromate 

 solution as before are treated as follows : Arsenic acid, 1 per 

 cent., half an hour ; osmic acid, 1 per cent., five to six hours. 



(3). Fresh tissues treated as follows : Gold chloride, 1 per 

 cent., half an hour; oxalic acid, 0'5 per cent.; warm in a 

 water-bath up to 36, allow to cool, and examine. 



Mount all these preparations in glycerin (balsam clears too 

 greatly). The methods only succeed completely during fine 

 sunny weather. 



637. Corpuscles of Golgi (CATTANEO, Arch. ital. de Biol., x, 

 1888, p. 337). The method here recommended is the arsenic- 

 acid method of Golgi that has been described above, 633, 

 sub voce " KUHNE " (4) . 



