342 SOME OTHER HISTOLOG1CAL METHODS. 



CHAPTER XXXI. 

 SOME OTHER HISTOLOGICAL METHODS. 



Connective tissues. 



682. Connective Tissue. S. MAYER (Sitzb. k. Akad. Wiss., 

 Ixxxv, 1882, p. 69) recommends, for staining fresh tissue, a 

 solution of 1 gramme of tf Violet B." (Bindschedler and 

 Busch, Bale) in 300 c.c. of 0*5 per cent, salt solution. In this 

 liquid connective tissue cells stain rapidly and energetically. 

 Elastic fibres and smooth muscle also stain, but of different 

 tints. 



FREEBORN (Amer. Mon. Hie. Journ., 1888, p. 231 : Journ. 

 Roy. Mic. Soc.y 1889, p. 305) recommends (for sections) 

 picro-nigrosin, made by mixing 5 c.c. of 1 per cent, aqueous 

 solution of nigrosin with 45 c.c. of aqueous solution of picric 

 acid. Stain for three to five minutes, wash with water and 

 mount in balsam. Connective-tissue fibres bright blue, nuclei 

 blackish, all the rest greenish-yellow. Sections may be after- 

 stained for five or six minutes in a mixture of 1 c.c. of satu- 

 rated alcoholic solution of eosin and 49 c.c. of alcohol. The 

 results are as before, except that the yellow colour is replaced 

 by red. 



DOQIEL (Anat. Am., 1887, p. 139 ; Zeit. f. wise. MM., 1887, p. 86) puts 

 tendons from the tail of the rat for some hours, days, or weeks into 

 Grenadier's alum-carmine. The tendon-bundles swell up and become trans- 

 parent, the cells are well stained, and elastic fibres well brought out. 



He stains subcutaneous connective tissue with concentrated solution of 

 fuchsin diluted with one volume of water. 



For RANVIER'S method of artificial oedemata for the study 

 of areolar tissue, see his Traite, p. 329. 



683. Fat. DEKHUYSEN (see FLEMMINO, in Zeit. f. wiss. Mik., 

 1889, pp. 39, 178) has discovered that fat that has been 

 stained black by treatment with chromo-aceto-osmic acid 

 (not with pure osmium)* is dissolved in the course of a few 



* The statement made 28, p. 19, line 9 from bottom, is therefore mis- 

 leading. 



