GOBLET CELLS. 351 



Thinner sections can be obtained if the agar-agar mass be imbedded in 

 paraffin by infiltration in the usual way, instead of pith. 



Instructions, too long to be abstracted here, are given for the preparation 

 of a suitable agar-agar mass. It may be obtained ready prepared from 

 Herrn Ko'nig, 29, Dorotheenstrasse, Berlin. Celloidin, and others of the 

 usual imbedding masses, were tried, but without success. 



For further details the English reader may consult the Journ. Roy. Mic. 

 Soc., 1888, pp. 313, 659. 



This is undoubtedly a valuable method, and is capable of extension to the 

 study of other animal fluids besides blood. 



696. HAYEM'S Methods. In conclusion, see the exhaustive 

 work of HAYEM, Du sang et de ses alterations anatomiques, 

 pp. 1035, with 126 figures. Paris, Masson, 1889. A report 

 of over twenty pages on this important work is contained in 

 Zeit.f. wiss. Mik., vi, 3, 1889, p. 330. It has reached me too 

 late to be abstracted here. 



Glands. 



697. Mucus Glands. It has already been stated that the 

 blue solutions of haematoxylin have a special affinity for mucin. 

 For the demonstration of mucus gland-cells the following pro- 

 cess is recommended by FLEMMING (Zeit. f. wiss. Mik., 1885, 

 p. 518) : Stain sections first with haematoxylin of Heideiihain, 

 and afterwards with haematoxylin of Delafield or Bohmer. 

 The mucus cells are shown stained violet. 



698. " Reticulum " of Mucus Cells. This was first demonstrated 

 by SCHIEFFERDECKER (Arch. /. mik. Anat., 1884, Hft. 3) by means of 

 anilin-green. This reaction has given rise to a long polemic between 

 SCHIEFFERDECKER and LIST, for which see Zeit. /. wiss. Mik., 1885, pp. 

 51, 222, 223. According to List, the reticula in question stain in an equally 

 specific way in methyl-green, in Bismarck brown, in nitrate of rosanilin (of 

 O'OOOl per cent., ten to fifteen minutes, see op. cit., iii, 3, 1886, p. 393), and 

 in List's double-stains ( 253, 254). Schiefferdecker, on the contrary, 

 maintain! that the reticula demonstrated by the methods of List are not 

 identical with those demonstrated by anilin-green. 



699. Goblet Cells (FLEMMING, Zeit.f. wiss. Mik., 1885, p. 519). 

 The contents of certain epithelium cells of the intestine and 

 other organs take a specific dark blue or violet stain on treat- 

 in cut with haematoxylin after fixation with osmic acid or an 

 osmium mixture. Staining preparations so fixed with safranin 

 or gentian-violet in the manner described in 102 and 103 



