KILLING ANNELIDA. 367 



of cocain gave the best results. Warm water gave him good 

 results for large species, such as those of Hydatina and 

 Brachionus. 



HAEDY (Journ. Roy. Mic. Soc., 1889, p. 475) recommends 

 thick syrup added drop by drop to the water. HUDSON (Ibid., 

 p. 476) mentions weak solution of salicylic acid. 



Annelida. 



733, Cleansing Intestine of Lumbricus (KUKENTHAL, Journ. 

 Roy. Mic. Soc., 1888, p. 1044). Put the animals into a tall 

 glass vessel which has been filled up with bits of moistened 

 blotting paper. They gradually evacuate the earthy particles 

 from the gut and fill it instead with paper. 



734. Killing Annelida. Lumbricus may be anaesthetised by 

 putting the animals in water with a few drops of chloroform. 

 In order to kill Criodrilus lacuum, COLLIN (Zeit. f. wiss. ZooL, 

 xlvi, 1888, p. 474) puts the animals into a closed vessel with 

 a little water, and hangs up in it a strip of blotting paper 

 soaked in chloroform. KUKENTHAL (Die mik. Technik, 1885 ; 

 Zeit. f. wiss. Mik., 1886, p. 61) puts annelids into a glass 

 cylinder filled with water to the height of 10 centimetres, and 

 then pours 70 per cent, alcohol to a depth of 1 to 2 centi- 

 metres on to the water. The animals will be found sufficiently 

 narcotised for fixation in from four to eight hours. For 

 Opheliadse he also employs O'l per cent, of chloral hydrate in 

 sea water. 



The Polychdsta sedentaria offer the difficulty of a complex 

 and very contractile branchial apparatus. They may some- 

 times be satisfactorily fixed by bringing them rapidly into 

 corrosive sublimate. De Castellarnau (1. c., p. 139) says that 

 SALVATORE Lo BIANCO obtains magnificent preparations of 

 Spirographis by this means. The species of Polychaeta 

 errantia that offer a contractile branchial apparatus, as Eunice 

 and Onuphis, may be treated in the same way. 



The greater part of the remaining forms of Polychaeta and 

 other marine annelids may be satisfactorily killed, according 

 to De Castellarnau, by the alcohol method of Eisig, 13. 



Very delicate pelagic forms, such as Alciope, Alciopina, 

 Vanadis, may be killed and fixed by iodised alcohol. SALVATORE 

 Lo BIANCO brings them for one or two minutes into a mixture 



