382 SOME ZOOLOGICAL METHODS. 



ti-n to twenty minutes with picro-carmine, wash with water and mount in 

 equal parts of glycerin and water). 



FOL (Lehrb., p. 102) fixes delicate marine Infusoria (Tintinnodea) with 

 the perchloride of iron solution 50 added to the water containing them, and 

 stains with gallic acid as directed 214, and states that this is the only 

 method that has given him good results, especially as regards the preservation 

 of cilia. 



KUNSTLEE (Journ. de Microgr., 1886, pp. 17 and 58). For Monadina. 

 Fixation by means of a drop of very concentrated osmic acid solution (a 

 gramme of osmium dissolved in only a few c.c. of distilled water). 



SCHEWIAKOFF (Morphol. Jahrb., xiii, 1887, p. 193). Fixation with 

 liquid of Flemming allowed to act for only a very short time and very 

 thoroughly washed out afterwards. Staining with alum carmine (or picro- 

 carmine, to be carefully watched, as it easily overstains). 



BRANDT'S Methods for Sphaerozoa, Fauna u. Flora d. Golfes v. 

 Neapel, 1885 ; see also Journ. Roy. Hie. Soc., 1888, p. 665. For some 

 forms, fixation for fifteen to thirty minutes in a mixture of 1 part 70 per 

 cent, alcohol, 1 part sea- water, and as much tincture of iodine as will impart 

 a distinctly yellow colour to the mixture. This is followed by washing out 

 with alcohol. For other forms, 0'4 to 1 per cent, chromic acid. For others, 

 5 to 15 per cent, solution of sublimate in sea-water. The best stain way 

 found to be aqueous ha}inatoxylin. 



