494 APPENDIX. 



16. Mayer's Solution. 



15 per cent, solution of sugar-candy 20 cc. 



Dihydropotassic phosphate o- i grm. 



Calcic phosphate o* i grm. 



Magnesic sulphate q- i grm. 



Pepsin 0-23 grm. 



1 7. Midler's Fluid. 



Bichromate of potash 25 grammes. 



Sodic sulphate 10 grammes. 



Distilled water i litre. 



18. Osmic Acid, Solution of. 



Best bought ready made in the form of i per cent, solu- 

 tion. 



The crystals are supplied in small tubes each containing 

 I gramme. Such a tube should be broken up in 100 c. c. 

 of distilled water, great care being taken to avoid contact 

 with the resulting vapour. 



This reagent should be kept in a black stoppered 

 bottle, perfectly in the dark. 



19. ParaflBn. 



See Appendix F. 



20. Pasteur's Solution. 



Potassium Phosph 20 parts. 



Calcium Phosph 2 „ 



Magnesium Sulphate 2 „ 



Ammonium Tartrate 100 „ 



[Cane Sugar 1500 „ ] 



Water 8376 „ 



10,000 parts. 



The sugar is to be omitted when Pasteur's fluid "without 

 sugar" is ordered. Pasteur himself used actual yeast 

 ash ; the above constituents give an imitation ash, which, 

 with the ammonium salt and sugar, answers all practical 

 purposes. 



