130 MASS. EXPERIMENT STATION BULLETIN 176. 



Having found that manganese is carried up into the tops of the plants 

 the following experiments were tried to determine if there was an increase 

 in the amount of manganese in the tops of clover grown on plots where 

 the poor vegetation was thought to be due to manganese. 



The first crop of clover analyzed was the same as that reported in 

 Bulletin No. 161. The tops only were analyzed, and the results were 

 based on dry matter. 



Table VH. — Milligram of Manganese Oxide (MW3O4) in 1 Ch-am of 



Clover. 



The clover from the limed portions of the plots shows very Uttle differ- 

 ence between the different plots. The plants from the unlimed portions 

 show a marked increase of manganese in those plots receiving sulfate of 

 ammonia. 



In the spring of 1915 samples of clover, grass, clover roots, and grass 

 roots were taken from the limed and unlimed portions of plot 5.^ From 

 the unlimed end two samples were taken, one of normal looking plants 

 and another of poor plants. The plants were brought into the laboratory 

 and the roots carefully washed free of soil, especial care being taken not to 

 break many of the finer roots. The tops were then cut from the roots, 

 and the clover separated from the grass, the same being done with the 

 roots. They were then dried at 75 degrees and ground. The tops were 

 then analyzed for iron, manganese and silica. The roots were only an- 

 alyzed for manganese as it is almost impossible to wash them entirely free 

 from soil which would invalidate the results fo'r iron and silica. 



1 Plot 5 is fertilized as follows: (NH4)2S04, dissolved boneblack, low-grade sulfate of potash. 



