CLEAVAGE OF PROTEINS 57 



blood pressure and delays the coagulation of 

 the blood. 



I took powdered pneumococcus cellular sub- 

 stance which had been prepared nearly seven 

 years before. Microscopic examination showed 

 the pneumococci as clearly defined and in as 

 perfect form as in a fresh preparation. Five 

 hundred milligrams of this powder was sus- 

 pended in 500 c.c. of salt solution, 10 c.c. of 

 chloroform added and kept at 37. After 

 twenty-four hours 10 c.c. of the opalescent su- 

 pernant fluid was administered to a guinea-pig 

 intravenously. Within two hours the animal's 

 temperature fell to 94, but recovery followed. 

 The same experiment repeated after 48 and 72 

 hours killed the animal within two hours with 

 the symptoms of subacute anaphylactic shock. 

 A like injection after six days killed within 

 three minutes with all the symptoms and post- 

 mortem findings of acute anaphylactic shock. 



It has been shown that the cholera bacillus 

 does not undergo ready autolytic cleavage in 

 vitro, but there is reason for suspecting that 

 this happens in the intestine of infected men, 

 since after death the bacillus is found only in 

 the intestinal canal, in some instances at least, 

 all the internal organs being sterile. 



Warden finds that the gonococcus early un- 



