72 POISONOUS PROTEINS 



suits followed when the incubation was made 

 with fluids obtained from an animal sensitized 

 three days previously with egg-white. (3) 

 When the fluids were obtained from animals 

 sensitized fourteen days previously to egg- 

 white, anaphylactic shock followed in all. (4) 

 With the conditions as in (3) except that the in- 

 cubation was prolonged to ninety minutes, the 

 effects were less marked. (5) With the condi- 

 tions the same as in (3) except that the incuba- 

 tion was done in a cold room the effects were 

 nil. (6) When the fluids were obtained from an 

 animal seventeen days after sensitization, ana- 

 phylactic shock resulted. (7) Filtration of the 

 fluids through hard fiber paper did not affect 

 the results. (8) Filtration of the serum and or- 

 gan extract through a Berkefeld V did not af- 

 fect the results. (9) Filtration after incuba- 

 tion did not affect the results. (10) When the 

 serum and organ extracts were heated to 56 

 for thirty minutes there were no effects. (11) 

 When the heated serum and organ extract were 

 activated by the addition of unheated serum 

 and extracts the effects were positive. (12) 

 When the serum and organ extracts of a 

 guinea-pig sensitized to egg-white were incu- 

 bated with horse serum there were no effects. 

 (13) The serum and organ extracts of guinea- 



