42 



APPLIED BACTERIOLOGY FOR NURSES 



gangrenous or for very foul wounds a more frequent 

 application should be made, as the active chlorine, on 

 which the action of the chloramines depends, is more 

 rapidly consumed by such wounds. 



Halazone is the name given by Dakin to a compound 

 allied to the preceding and designed especially for the 

 emergency disinfection of drinking-water. Dakin's ex- 

 periments indicate that a concentration of 1 : 300,000 

 will sterilize an ordinarily heavily contaminated water in 

 about thirty minutes. Halazone has been prepared in 

 the form of tablets made with sodium carbonate or with 

 dry borax. 



Demonstration. — The teacher should inoculate a loopful from a 

 twenty-four-hour broth culture of typhoid (or colon) bacilli into the 

 following tubes: and make subcultures from each of these tubes at 



10 cc. 

 Carbolic 



10 c.e. 



J- J 000 



Biehlorld 



locc. 



plain. 



Sterile 



Water 



Fig. 18. 



the end of one minute or five minutes by inoculating a loopful from 

 these tubes into plain sterile broth. 



Instead of making broth subcultures, agar poured plate cultures 

 can be made. 



