HYDROGEN ION CONCENTRATION AND PROTEOLYSIS. 157 



made up to 500 cubic centimeters. This was shaken for thirty- minutes. 

 These two portions were then mixed thoroughly and treated in the same 

 manner as Medium III., with the following additional physical determi- 

 nations, namely, osmotic pressure, viscosity, surface tension and specific 

 conductance. Such determinations were made by taking samples after 

 subjecting the mixture to the autoclave. In addition to these, the total 

 nitrogen was determined by the Kjeldahl method. The results will be 

 given in Table I. 



