256 GENERAL THERAPEUTICS FOR VETERINARIANS 



nodular swellings of the lymph glands up to the size of a hazel- 

 nut may be felt; later, these rupture; on post-mortem examination, 

 purulent lymph glands and glanders abscesses are found, the latter 

 especially in the testicles (glandular orchitis); also glandular 

 nodules in the spleen, liver and limgs. A negative result from the 

 inoculation does not exclude the presence of glanders. When the 

 result is positive, a diagnosis of glanders is only justified when the 

 bacillus mallei is also demonstrated in cultures (pseudo-glanders!). 

 Other inoculation animals are the horse and the ass, the latter 

 contracting acute glanders and usually dying in about 8 days; 

 also cats, which, after subcutaneous (dorsal) inoculation, present 

 symptoms similar to those exhibited by guinea-pigs. Concerning 

 injection with mallein, see page 260. 



4. Tuberculosis. — The best inoculation animal is the guinea- 

 pig. After the usual intraperitoneal inoculation (milk), numerous 

 fresh tubercles of the size of millet seed appear in about three 

 weeks upon the peritoneum and in the spleen, liver^ and lymph 

 glands. Following subcutaneous injection (mucus from the 

 lungs), a purulent ulcer develops at the point of injection and the 

 neighboring lymph glands become swollen; after 3 to 4 weeks 

 numerous tubercles form in the internal organs. After intra- 

 muscular inoculation (rapid inoculation), the neighboring lymph 

 glands are transformed in ten days into firm nodules of the size 

 of small peas, which may be extirpated and examined microscopic- 

 ally. Intramammary inoculation and subcutaneous injection with 

 simultaneous crushing of the lymph glands are followed in five 

 days by the appearance of nodular swellings in the glands con- 

 cerned. Test inoculations of guinea-pigs is the most certain method 

 of clinically diagnosing tuberculosis. Concemmg inoculation with 

 tuberculin, see page 258. 



Fowl Cholera. — A pigeon is inoculated subcutaneously with 

 blood from a dead bird. A drop of blood from the dead bird is 

 introduced under the skin of the breast of the pigeon by means of 

 a lancet to the depth of a millimetre. If cholera is present, the 

 musculature beneath the point of inoculation becomes necrotic 



