LOUPIXG-ILL. 569 



June 11, Temperature 105° F. 



12. Temperature 103° F. Results negative. 



16. Sheep No. 3 inoculated, 1 p.m. Temperature 103° F. 



17. Temperature 103° F. 



1 8. Temperature 102-4° F, 



19. Temperature 102-6° F. 



20. Temperature 102° F, Results negative. 



16. Sheep No. 4 inoculated with tick cultivation in egg albumen, 1 p.m. 

 Temperature 103-4° F. 



17. Temperature 103° F. 



18. Temperature 103° F. 



19. Temperature 103-2° F. 



20. Temperature 104° F. Results negative. 



20. Tup No. 5 inoculated, 4 30 P.M. Temperature 103° F. 



21. Temperature 105° F. 



22. Temperature 103° F. 



23. Temperature 103° F. 



24. Temperature 103-6° F. 



25. Temperature 103° F. Results negative. 



29. Sheep No. 6 inoculated in vein. Temperature 104° F. 



30. Temperature 104° F. 



July 1. Temperature 103-8° F. Results negative. 



June 29. Sheep No. 1 reinoculated in hack (subcutaneously). Temperature 

 103° F. 

 ,, 30. Temperature 103-8° F. 

 July 1. Temperature 103° F. Results negative. 



The attempt to induce the disease by placing the ticks upon 

 healthy sheep, and by inoculations with the organism cultivated 

 from ticks, having proved unsatisfactory, and the season being 

 too far advanced for further experiments, I determined to adopt 

 another line of procedure during the season 1895. I may here 

 state that all the experiments with living ticks had hitherto 

 given negative results : ticks, except small ones and those which 

 had not yet become adherent to the sheep, could not be induced 

 to cling to a fresh host ; no matter how many fairly or fully 

 grown ticks were placed on a sheep, they would disappear in a 

 few hours. 



I then determined to send sheep during the following year to 

 tick-infested districts ; to muzzle these sheep in order to prevent 

 them from grazing ; to feed and water them indoors with food 

 sent from the College ; and to allow them to remain in the 

 infested district until infested with the ticks, then have them 

 returned to the College for examination and further study. 



"April 21, 1896.— We visited Mr. Hamilton, Leithen Hall, 

 and an adjoining farm, occupied and owned by Mr. Carruthers. 



