HEMOLYTIC PROPERTIES OF HELODERMA VENOM. 



149 



With a constant relatively small quantity of lecithin relatively very large 

 doses of venom are required in order to cause hemolysis much larger doses 

 than were required for the hemolysis of ox and guinea-pig corpuscles; while 

 with a constant dose of venom more lecithin is required for the hemolysis of 

 sheep than of ox corpuscles. In a combination of lecithin and venom more of 

 either of these two substances is required for the hemolysis of ox than of 



guinea-pig corpuscles. 



DOG CORPUSCLES. 



2 c.c. of 5 per cent suspension of dog corpuscles. 



In spite of the relatively small quantity of venom used, the addition of 

 one-quarter of the hemolytic dose of lecithin to the venom is sufficient to cause 

 hemolysis of dog corpuscles. 



When the quantity of venom used was variable and only small quantities 

 of lecithin were used, very small quantities (0.006 mg.) of venom were sufficient 

 to cause partial hemolysis of the dog corpuscles, indeed somewhat less than was 

 necessary to cause partial hemolysis of ox corpuscles (0.01 mg.), and very much 

 smaller quantities than caused hemolysis with sheep corpuscles (0.2 c.c. fresh 

 venom, none with 0.2 mg. dry venom). 



2 c.c. of 5 per cent suspension of dog corpuscles. 



RABBIT CORPUSCLES. 

 2 c.c. of 5 per cent suspension of rabbit corpuscles. 



The reaction of the corpuscles to the venom and lecithin varied consider- 

 ably. In certain cases, indeed, in most cases, the corpuscles were resistant to 



