154 



THE VENOM OF HELODERMA. 



SHEEP CORPUSCLES. 



Sheep corpuscles which react to sodium oleate in the same manner as ox 

 corpuscles, being dissolved by 0.002 gm. of sodium oleate, were not hemolyzed 

 by the mixtures of venom and sodium oleate used in our experiments. We 

 have tested 0.01 mg. of sodium oleate plus 1 mg. of venom, but found no hemo- 

 lysis. It is apparent that sheep corpuscles are more resistant to the action of 

 venom plus sodium oleate than are the ox corpuscles. 



DOG CORPUSCLES. 



Dog corpuscles are hemolyzed by 0.4 mg. of sodium oleate but not by 0.2 

 nag. of this substance. The smaller quantity, namely, 0.2 mg., of sodium 

 oleate is able to produce hemolysis after the addition of venom. 



1 c.c. of 5 per cent suspension of dog corpuscles. 



With dog corpuscles sodium oleate has only a slight activating power for 

 the venom, as it is necessary to add half or more than half the hemolytic dose 

 of sodium oleate to the venom in order to produce hemolysis. 



RABBIT CORPUSCLES. 



Rabbit corpuscles are hemolyzed by 0.5 mg. of sodium oleate and the 

 quantity of this substance which must be added to the heloderma venom in 

 order to produce hemolysis is more than half of this quantity. Again the acti- 

 vating property of the sodium oleate is very slight. 



GUINEA-PIG CORPUSCLES. 



Guinea-pig corpuscles are slightly hemolyzed by 0.125 mg. of sodium oleate. 

 In the mixture of venom and sodium oleate about half as much sodium oleate 

 is necessary to hemolyze the corpuscles. 



1 c.c. of 5 per cent suspension of guinea-pig corpuscles. 



Control, 0.125 mg.; sodium oleate, X. 



