HEMOLYTIC PROPERTIES OF HELODERMA VENOM. 



157 



the heated serum show any activating power. We may therefore conclude 

 that serum heated above 62 C. does not become an activator in the case of 

 heloderma venom. 



Dog serum was found to activate the heloderma venom. With ox and 

 sheep corpuscles the activating property of the dog serum is slight, since the 

 quantity of serum when combined with venom necessary to completely hemo- 

 lyze either ox or sheep corpuscles will by itself cause slight hemolysis of both 

 kinds of corpuscles. 



2 c.c. of 5 per cent suspen- 

 sion of sheep corpuscles. 



2 c.c. of 5 per cent sus- 

 pension of ox corpuscles. 



Amt. of 

 venom. 



mg. 

 0.2 



0.05 

 0.00 



1 Venom + 



| 0.1 c.c. dog 



serum. 



Total. 



Total. 



Total. 



X 



In the case of dog corpuscles the results are more striking, since a quantity 

 of dog serum equal to half the hemolytic dose for dog corpuscles was sufficient 

 to activate the heloderma venom. 



1 c.c. of 5 per cent suspension 

 of heloderma corpuscles. 



Rabbit corpuscles appeared to be slightly more resistant than the dog 

 corpuscles to the hemolytic effect of venom and dog serum combined. The 

 rabbit corpuscles, like dog corpuscles, were hemolyzed by 0.2 c.c. of dog serum. 

 When the dog serum and venom were combined 0.1 c.c. of the former was suffi- 

 cient when added to 0.1 mg. of venom to cause hemolysis. If only 0.05 mg. of 

 venom was added to the dog serum no hemolysis took place. 



Guinea-pig corpuscles were completely hemolyzed by 0.2 c.c. of dog serum 

 alone. When venom and dog serum were combined 0.1 c.c. of the latter and 

 0.02 mg. of venom were sufficient to cause moderate hemolysis, while 0.1 c.c. of 

 serum and 0.06 mg. of venom caused complete hemolysis of guinea-pig cor- 

 puscles. 



Heloderma corpuscles were also hemolyzed by venom and dog serum. 

 While 0.2 c.c. of dog serum did not hemolyze these corpuscles, this amount of 

 serum was able when combined with the venom to cause hemolysis of the helo- 

 derma corpuscles. Smaller quantities of dog serum did not activate the venom 

 for these corpuscles. 



