HEMOLYTIC PROPERTIES OF HELODERMA VENOM. 



165 



found that this immune serum inhibited hemolysis in the same degree as did the 

 serum of a normal rabbit; the immune serum had no greater protective power 

 than the normal serum. 



We also tested the inhibitory action of the serum of a rabbit that had been 

 immunized against dog serum and dog corpuscles. At the same time parallel 

 experiments were carried out in which normal rabbit serum was used to inhibit 

 hemolysis. 



Mixture of venom 0.1 mg. and lecithin 0.05 mg. and 2 c.c. of a 5 per cent 

 suspension of rabbit corpuscles. 



In this experiment the serum of the rabbit immunized against dog serum 

 and corpuscles was distinctly more protective for the rabbit corpuscles than the 

 normal serum. 



CORPUSCLES OF RABBIT IMMUNIZED AGAINST HELODERMA VENOM. 



The corpuscles of a rabbit, immunized against heloderma venom, were 

 washed; a 5 per cent suspension was prepared in the usual manner and the 

 hemolytic influence of venom combined with various activators was tested. 

 At the same time the influence of the venom-activator mixture was tested on 

 corpuscles of normal animals. 



When lecithin was added to venom in graded quantities, or when variable 

 quantities of venom were added to lecithin, the corpuscles of the immunized 

 animal showed slightly less hemolysis than those of the normal animal. 



Venom 0.1 mg. plus 2 c.c. of a 5 

 per cent suspension of corpuscles. 



Lecithin, 0.05 mg. 



With heated dog-serum as an activator, the corpuscles of the immunized 

 animal showed slightly more hemolysis than the corpuscles of the normal ani- 

 mal; in view of these slight differences and apparent contradictions it seems 

 probable that no marked differences exist between the resistance of the corpus- 

 cles of immunized rabbits and of normal rabbits to the hemolytic action of 

 heloderma venom and various activators. 



