198 THE VENOM OF HELODERMA. 



trolling this were similar tubes with increasing and decreasing doses of com- 

 plement. 



(1) 0.5 c.c. serum and 0.5 c.c. venom and .15 c.c. complement. 



(2) 0.5 c.c. serum and 0.5 c.c. venom and .12 c.c. complement. 



(3) 0.5 c.c. serum and 0.5 c.c. venom and .10 c.c. complement. 



(4) 0.5 c.c. serum and 0.5 c.c. venom and .08 c.c. complement. 



(5) 0.5 c.c. serum and 0.5 c.c. venom and .06 c.c. complement. 



Incubate for 2 hours at 37 C., then add 1 c.c. washed erythrocytes and 2 units of 

 amboceptor and incubate again for 1 hour at 37 C. 



Result: Result: 



(1) Complete hemolysis. (4) No hemolysis. 



(2) Complete hemolysis. (5) No hemolysis. 



(3) Partial hemolysis. 



CONCLUSIONS. 



In all four tests made and using both systems, no true fixation of comple- 

 ment could be determined. In no case was more complement absorbed than 

 the sum of that fixed by the venom and serum alone. By this method, there- 

 fore, the presence of an antibody can not be demonstrated. 



BIBLIOGRAPHY. 



1. REDT, F. Observationes de Viperis scriptae literis ad gener. dominum Laurentium 



Majalotti 18 Amsterd., 1675. (Quoted in "Researches on Rattlesnakes, " by Weir 

 Mitchell Washington, 1860.) 



2. FONTANA. Recerches fisiche sopra el vileno della vipera. Lucca, 1767. Translated by 



Skinner. London, 1787. 



3. FONTANA, F. Abhandlung iiber das Vipera gift. Berlin, 1787. 



4. PHISALIX and BERTRAND. Compt. rend. Soc. de Biol., 1893. 



5. WEIR MITCHELL. Researches upon the venom of rattlesnakes. Smithsonian Contribu- 



tions to Knowledge, 1860. 



6. BOURNE. Proc. Royal Society, London, 1887. 



7. FAYRER. The Thanatophidia of India, London, 1874. 



8. COOKE and LOEB. Proc. Soc. Exp. Biol. and Med., 1908, v, 104. 



9. PHISALIX and BERTRAND. Compt. rend. Soc. de Biol, 1893; also Compt. rend. Acad. Sci., 



1893. 



10. BERNARD, C. Lemons sur les effets des substances toxiques, Paris, 1857. 



11. PHISALIX and BERTRAND. Archiv de Physiol, 1894. 



12. LEYDIG. Archiv fur mikrokopisches Anatomic, 1873. 



13. REICHL. Morphologisches Jahrbuch., 1883. 



14. BLANCH ARD. Compt. rend. Soc. de Biol., 1884. 



15. JOURDAIN. Compt. rend. Acad. Sci., 1894. 



16. PHISALIX and BERTRAND. Compt. rend. Soc. Biol., 1893; also Compt. rend. Acad. Sci., 



1893. 



17. PHISALIX and BERTRAND. Compt. rend. Acad. Sci., 1894; also Compt. rend. Soc. Biol., 



1894. 



18. CALMETTE, A. Venoms; trans. E. E. Austen, London, 1908. 



19. FLEXNER and NOGUCHI: Jour. Path, and Bact., 1903. 



20. NOGUCHI. Snake Venom, Washington, D. C., 1909. 



21. FRASER. Proc. Roy. Soc. Edinb., 1895, June 3. 



22. METSCHNIKOFF. Immunity of infectious disease, Cambridge, 1905. 



23. BRODET and GENGOU. Ann. de 1'Instit. Pasteur, xxv, No. 5. 



