222 THE VENOM OF HELODERMA. 



1 injected with a quantity corresponding to 3| lethal doses lived somewhat 

 longer. Very little venom had, therefore, been adsorbed, and accordingly we 

 find that a mouse injected with 2 c.c. of the residue of this rabbit-liver venom 

 mixture survived. 



Of 3 mice injected with the supernatant fluid from a mixture of fresh 

 venom and rabbit kidney, 2 lived longer than the controls, and a third died as 

 soon as the control. Little or no adsorptive effect of rabbit kidney was there- 

 fore evident. Similar results were obtained with the supernatant fluid when a 

 solution of dry venom had been mixed with rabbit kidney, and, furthermore, 

 one mouse injected with the residue from this mixture survived. Considerably 

 less than 70 per cent of the venom is adsorbed by rabbit liver and kidney. 



Nine mice injected with the supernatant fluid from a venom dog-brain 

 mixture died as soon as their controls. Since even by prolonged centrifugation 

 it was impossible to throw down some of the finest particles of the dog-brain 

 venom emulsion,* we filtered this mixture through a Berkefeld filter in order to 

 obtain fluid free from brain tissue; 2 animals injected with a quantity of the 

 filtrate corresponding to 13J lethal doses and 6f lethal doses, respectively, 

 lived longer than their controls, while 1 injected with a quantity corresponding 

 to 3| lethal doses survived the injection. These results make it probable that 

 the lethal effect of the non-filtered supernatant fluid was in part due to the very 

 fine particles of brain tissue present in this fluid. These fine particles of brain- 

 tissue which were removed by filtration had evidently adsorbed considerable 

 quantities of venom and when injected into mice gave up the venom insufficient 

 quantities to cause the death of the animal. We may therefore conclude that, 

 under the conditions of our experiments, dog brain adsorbs almost 70 per cent 

 of the venom. We found that 2 of 3 mice injected with residue from dog-brain 

 venom mixture survived; 1 died. It seems, therefore, very probable that the 

 fine particles which remained in the supernatant fluid had adsorbed very large 

 proportions of the venom; it still remains to be seen whether some venom 

 had been removed from the brain-pulp during the process of washing. 



Six mice were injected with the supernatant fluid from a venom dog-liver 

 mixture; 5 died as soon as the controls; 1 injected with a quantity correspond- 

 ing to 6f lethal doses survived. Six mice injected with the supernatant fluid 

 from dog-kidney venom mixture all died as soon as their controls. It is there- 

 fore evident that dog kidney, like dog liver, adsorbed no venom, or almost none. 



Two mice were injected with residue from the dog-liver venom mixture 

 and 2 with residue from dog-kidney venom mixture; all survived. These 

 results are in accord with the results obtained in the case of the supernatant 

 fluid and confirm the conclusion that neither of these organs adsorbs appreci- 

 able quantities of venom. 



Two series of six mice each, injected with the supernatant fluid from a 

 mixture of venom with either washed dog-erythrocytes or the stroma of these 

 cells, died as soon as their controls. Notwithstanding these results, injection of 



*The supernatant fluid from the brain-venom mixtures contained fine particles of brain substance, not only in 

 the case of dog, but of all other species as well; although more pronounced in the case of dog brain. 



