Die Teclmik der Grewebskiiltur in vitro. 



Von Alexis Carrel und Montrose T. liurrows, New York. 



(Aus dem Laboratorium des Rockefeller Institutes für medizinische Forschung.) 



Man kultiviert ein Gewebe oder ein Organ, indem man unter ge- 

 wissen Bedingungen Fragmente dieses Gewebes oder (3rganes in einen 

 passenden Kulturnährboden einimpft. Diese neue Methode, deren Resultate 

 zum Teil schon beschrieben worden sind ^), ist zahlreicher Anwendung fähig. 



*) Harrison, Observations on living developing nerve fibres. Proc. of the Soc. for 

 Exp. Biolog. and Med. 1907. p. 140. — Embryonic transplantation and the development of 

 the nervous System, the Harvey lectures 1907—1908. — The outgrowth of the nerve fibre as 

 a mode of protoplasmic movement. Journal Exp. Zool. 1910. Vol. 9. p. 787. — Burrows, The 

 growth of tissues of the chick emlu'yo outside of the animal body with special reference to 

 the nervous System. Journal of Exp. Zool. 1911. Vol. 10. p. 63. — Culture des tissus d'embryon 

 de poulet et specialement culture des nerfs de poulet en dehors de l'organisme. C. R. Soc. de 

 Biologie. 1910. p. 291. — Carrc? and Burrows, Cultivation of adult tissues and organs out- 

 side of the body. Journ. Am.Med. Assoc. 1910. p. 1379. — Cultivation of sarcoma outside of 

 the body. Journ. Am. Med. Assoc. 1910. p. 1554. — Human sarcoma cultivated outside of 

 the body. Journ. Am. Med. Assoc. 1910. p. 1732. — Artificial Stimulation and inhibition 

 of the growth of normal and sarcomatous tissues. Journ. Am. Med. Assoc. 1911. p. 32. — 

 Cultivation of tissues in vitro and its technique. Journal of Exp. Med. 1911. Vol. 13. 

 p. 387. — Cultivation in vitro of the thyroid gland. Journ. of Exp. Med. 1911. Vol. 13. 

 p. 416. — On the physico-chemical regulation of the growth of tissues. Journ. of Exp. 

 Med. 1911. Vol. 13. p. 562. — Cultivation in vitro of malignant tumors. Journ. of Exp. 

 Med. 1911. Vol. 13. n. 571. — La culture des tissus adultes eu dehors de l'organisme. 

 C. R. Soc. de Biol. 1910. p. 293. — Culture de substance renale en dehors de l'organisme. 

 C. R. Soc. de Biol. 1910. p. 298. — Culture de moelle osseuse et de rate. C. R. Soc. 

 de Biol. 1910. p. 299. — Cultures primaires, secondaires et tertiaires de glande thyroide 

 et culture de peritoine. C. R. Soc. de Biol. 1910. p. 329. — Culture de sarcome en dehors 

 de l'organisme. C. R. Soc. de Biol. 1910. p. 332. — Culture in vitro d'un sarcome humain. 

 C. R. Soc. de Biol. 1910. p. 367. — Seconde generation de cellules thyroidiennes. C. R. 

 Soc. de Biol. 1910. p. 365. — A propos des cultures „in vitro" des tissus de mammi- 

 feres. C. R. Soc. de Biol. 1911. p. 3. — La culture des tissus in vitro. La Presse Medi- 

 cale. 1911. p. 209. — Carrel, Abnormal forms of life and their applicatious. American 

 Philosophical Society, Philadelphia 1911. — Rutli, Cicatrisation of wounds in vitro. 

 Journ. of Exp. Med. 1911. Vol. 13. p. 422. — The influence of distilled water on the 

 healiüg of skiu wounds iu the frog. Journ. of Exp. Med. 1911. Vol. 13. p. 559. — 

 Cicatrisation de plaies cutanees en dehors de l'organisme. C. R. Soc. de Biol. 1911. 

 pag. 253. — Jollji, A propos des Communications de MM. Alexis Carrel et Montrose 

 T. Burroivs sur la culture des tissus. C. R. Soc. de Biol. 1910. p. 470. — Observations 

 ä l'occasion de la communication de MM. Carrel et Burrows. C. R. Soc. de Biol. 

 1911. p. 4. — Lambert and Hau es, Growth in vitro of the transplantabie sarcomas of rats 



