TUBERCULOSIS. 435 



ously with these 2 cultures from children died of generalized tuber- 

 culosis. Two calves weighing more than 300 pounds each were 

 inoculated subcutaneously with these virulent human cultures, and 

 as a result developed generalized tuberculosis. A yearling heifer 

 inoculated with 1 of the cultures showed generalized tuberculosis 

 when killed three months after inoculation. Both the cattle and 

 the hogs had been tested with tuberculin and found to be free from 

 tuberculosis before the inocidations were made. It is important 

 to observe in this connection that 2 out of 4, or 50 per cent, of the 

 cultures obtained from cases of generalized tuberculosis in children 

 proved virulent for cattle. 



Mohler, working in the Pathological Division, Bureau of Animal 

 Industry, obtained 3 very virulent cultures of tubercle "bacilli from 

 the human subject. A goat inoculated subcutaneously with 1 of 

 these cultures died in 37 days with miliary tuberculosis of the lungs 

 involving the axillary and prescapular glands. This bacillus was 

 obtained from the mesenteric gland of a boy. Of still greater 

 interest is a bacillus isolated by Mohler from human sputum. A 

 goat inoculated subcutaneously with a culture of this germ died in 

 95 days of pulmonary tuberculosis. A cat inoculated in the same 

 manner died in 23 days of generalized tuberculosis. A rabbit simi- 

 larly inoculated died in 59 days of pulmonary tuberculosis. An- 

 other rabbit inoculated with a bovine culture for comparison lived 

 10 days longer than the one inoculated with this sputum germ. 

 Mohler also inoculated subcutaneously a l-year-old heifer with a 

 culture derived from the tuberculosis mesenteric gland of a boy 4 

 years of age. This culture was always refractory in its growth under 

 artificial conditions, and the bacilli were short, stubby rods, corre- 

 sponding in appearance to the bovine type. At the autopsy, held 

 127 days after the inoculation, the general condition was seen to be 

 poor and unthrifty, and large, hard tumors were found at the points 

 of inoculation. On the right side the swelling measured 3^ by 5 

 inches, and the corresponding lymph gland was 2J inches long by 

 If inches in diameter. This gland contained numerous calcareous 

 foci: one at the apex was an inch in diameter. The lesions on the 

 left shoulder of the animal were very similar to those found on the 

 right side, but the dimensions of the tumor were slightly less. The 

 lungs presented • an irregular mass of tuberculous nodules, and 7 

 or 8 grapelike nodules were seen on the parietal pleura. Bronchial 

 and mediastinal Ij^mph glands contained numerous tuberculous foci, 

 and the pericardium, peritoneum, spleen, and liver also were affected. 



In order to throw some light, if possible, upon the morphological 

 constancy of the different types of tubercle bacilli, Mohler made 

 comparative studies of bacilli from various sources, and which had. 

 been passed through various species of ainmals, by making the cul- 



