jo THE LOT OP PASTEUB 



or ^ -of tl. D the egg into those small 



j nps, all eggs should putrefy equally, whereas they do not." 



• end Of M- liayon's thesis— which had not taken so 

 long as Banlin'i I re, only three years— we find I 

 following conclusion : " I etion in I ggs is con ith 

 th* multiplication of beings which are bac- 



D in contact with air and vibriones when away from 



from that {>oint of view, do not 

 il law discovered by M. Pasteur." 



• .r's influence was DO* B] ig beyond the Labora- 



hyaiological Chemistry, ai the small laboratory at the 

 trmale was called. 

 In the treat he had published in 1862, criticizing th« 

 doctrine of spontaneous generation, he had mentioned, among 

 or£ai duced by urine in putrefaction, the exist- 



e of a toroJacea in very small-grained chaplets. A physician, 

 Tranbe, in 1864, had demonstrated that Pasteur was right 

 in thinking that aininoniacal fermentation was due to this 

 . who- | -Tties were afterwards studied with in- 



j M. Van Tieghem, a former student of the Ecole 

 . who had inspired Pasteur with a deep affection. 

 :r. in his turn, completed his own observations and 

 assured himself thai this little organized ferment was to be 

 found in every case of ammoniacal urine. Finally, after prov- 

 ing thai boracic acid impeded the development of that am- 

 moniacal ferment, he raj I to M G-nyon, the cel< brated 

 -• of boracic acid for washing out the bladder; 

 M. • advice into practice with success, and attri- 

 credil of it to Pasteur. 

 In a letter writi I of 1673, Pasteur wrote :" How 

 1 n I Bad enough health and sufficient knowledge to throw 

 I soul into the experimental study of one of 

 ■ I' I nsid. red that his studies on 

 f, ' r: would lead him in that direction; he thought 

 i it should be made evident \));>a every serious altersv- 

 lue to the micro-organisms which find in 

 f l»qu am favourable to their develop] when 

 lid \» that— in eontri to the I as by 

 those air is are looked upon ns spontaneous, in- 

 liquids, and depending on their .and 

 ,!ir cause of 1 ss is not interiof but 

 lid indei d be d« loctrine of men 



