while borax carmine gave an excellent stain when used after 

 acetic acid or sublimate-acetic, but was of no value for ob- 

 jects fixed in Perenyi's fluid. 



I have made use of Patten's method of orientation 

 (21) to great advantage, and have found it of invaluable as- 

 sistance in manipulating very small buds, which I was enabled 

 to cut with accuracy in any plane desired. 



In studying the bud-development of Perophora I have 

 employed serial sections of all the stages in the sagittal, 

 frontal and transverse planes, and also a complete series, 

 throughout the entire development, of buds mounted in various 

 positions as total preparations. 



The sections were cut from 3 to S/*- in thickness on 

 a Thome microtome, and all the drawings were made with the 

 aid of a Zeiss camera lucida. 



I take much pleasure in acknowledging my indebted- 

 ness to Prof. Brooks, for the kindly interest with which he 

 has follov/ed my work, and for valiiable assistance given me. 

 I also desire to express in this place my great appreciation 

 of the many courtesies extended to me by the late Colonel 

 Marshall MacDonald at the Station of the 'J. S. Pish Gommis- 



A preliminary account of some of my results '.vas 

 published in the Johns Hopkins University Circulars, No. 119, 

 June, 1895. 



