VARIATION OF SALMONELLA PULLORUM 



17 



Table 3.- 



-REACTIONS OF VARIANTS WITH POSITIVE AND NEGATIVE PULLORUM SERA 



in different concentrations of NaCI. — Continued. 



Remarks* 



Muddy type of agglutination 

 Muddy type of agglutination 

 Fine clumping in first tube 



No agglutination 

 No agglutination 

 Definite clumping in first 2 tubes 



Very fine clumps in tubes 1 and 2 



Muddy type of agglutination 

 Very fine agglutination 

 Fine agglutination in first 5 

 tubes, muddy in the sixth 



Muddy type of agglutination 

 Very fine agglutination in first 



3 tubes 

 Clumps decrease in size with dilu- 

 tion 



Muddy type of agglutination plus 



settling 

 Very fine agglutination, difficult 



to distinguish from true settling 

 Difficult to determine amount of 



settling and agglutination 



Loose type of clumping 

 Fine clumps 



Very fine agglutination 

 Fine agglutination 



*Refer to legend of Table 2 for explanation of symbols. 



The agglutinating response of the four antigens approached the normal more 

 closely in Table 3 than in Table 2. It is likely that the organisms may have been 

 altered slightly in composition during the interval between the two tests. Organ- 

 isms which exhibit variation, especially in their antigenic structure, apparently 

 may vary in the degree and type of reactions from time to time. 



The agglutination titres were determined in the host in five cases from which 

 the organisms were isolated. In every instance the organism was capable of 



