VARIATION OF SALMONELLA PULLORUM 



27 



Table 4. — Remarks concerning colonial and cellular morphology of 

 isolated variants. — continued. 



Original Variant 

 Strain No. 



No. 



Culture 

 Medium* 



Colonial and Cellular Morphology 



Experiment No. Ill 



P. A. Colony similar to VHI-a, Expt. II. See Figure 13. 



P. A. / Colony has granular surface, appendages projecting from edge, 

 \moves partly in mass; cells are long and filamentous. 



P. A. / Colony type similar to XH-a of Expt. II. Cells slightly pleomor- 

 phic. 



P. A. / Colony type similar to IV-b of Expt. I. Cells — ■ a few bizarre 

 \ forms observed. 



P. A. Colony type similar to VHI-a of Expt. II. 



P. A. Colony type similar to Vlll-a of Expt. II. See Figure 13. 



Experiment No. IV 



f Colony morphology similar to normal type. Occasionally a rough 

 L. A. \ beveled edce is observed. Interior is smooth and homogeneous. 

 { Cells— bizarre forms numerous. 



L. A. J Colony morphology similar to normal type, cells slightly pleomor- 

 \phic. 



Experiment No. V 



P. A. < Colony type similar to VIII-a of Expt. II. Cell morphology 

 \ slightly pleomorphic. 



P. A. f Colony surface inclined to be slightly rough and irregular at 

 times, with faint convolutions; edge entire and structure occasion- 

 ally granular; cells pleomorphic. 

 | Colony similar to VIII-a of Expt. II, but appears to be some- 



P. A. | what butyrous in consistency and more dense. Cells long, ap- 

 proaching short filaments, 

 f Colony has tendency to vary in its morphology. While resemb- 



P. A. -(ling the smooth type, it occasionally will produce rough colonies. 

 [Cells same as in Variant B. 



Il-b 

 X-c 



A 

 A-l 



B 



B-l 



lV-a D-l 



IV-a D-2 



XIV 



L. A. 



L. A. I 



L. A. 



L. A. I 



Experiment No. VI 



Colony has irregular edge, may appear curled; peripheral portion 

 thin and frequently markedly convoluted and filamentous. Central 

 portion appears raised and dense, at times filamentous. Surface 

 appears dull, consistency butyrous. Young colonies appear very 

 irregular, filamentous, and granular. Older colonies may resemble 

 lhe Medusa-head colony and attain a diameter of 1.5 cm. Cells 

 vary in length from the normal to long filamentous types. See 

 Figures 22, 23, 24, and 25. 



The variant when first placed on liver infusion agar produced a 

 very irregular colony possessing numerous projections. The 

 structure might be entirely granular, semi-granular, or entirely 

 filamentous. As the colony grew larger the angular portions were 

 filled in. Many cells of these colonies weie thick filaments of 

 various lengths. Other bizarre forms of cells were also observed. 

 Granular types were seen frequently, especially in older cultures. 

 The colony appeared dull and could be removed in mass. See 

 Figures 26, 27, and 28. This colony type has reverted almost 

 completely to smooth form. 



This variant followed practically the same course of leversion, 

 but not as completely, as D-l of Expt. VI. 



Young colonies exhibit numerous projections. The structure of 

 the projections and interior of the colony appears striated. Older 

 colonies appear less angular, but have an irregular outline, con- 

 voluted and striated periphery with a raised dense center. Isolated 

 colonies may attain a diameter of 1 cm. The cellular types in the 

 small striated colonies consisted largely of filamentous forms. Older 

 colonies contain cells of various sizes. See Figures 29 and 30. 



*P. A. — Plain meat extract agar. 

 L. A. — Liver infusion agar. 



