THE WHOLE BLOOD TEST FOR PULLORUM DISEASE 15 



On December 10 and 11, 1934, 976 birds were tested and revealed ten reactors. 

 The electrically equipped testing apparatus was used on this and subsequent 

 tests. The blood of two birds (99100 and 92237) gave delayed reactions. One, 

 92237, reacted in a similar manner on the first test, but did not react on the second 

 test. Three reactors (99255, 99017 and 99087) were submitted to the laboratory. 

 Bird number 99017 gave a delayed reaction with the whole blood method on the 

 second test. A small group of birds, approximately 80 in number, which had 

 passed two negative tests and were confined in a separate building were not tested 

 at this time. 



On January 14 and 15, 1935, the entire flock, consisting of 989 birds, was tested 

 and revealed four reactors (99052, 99130, 99239 and 99302). Four birds (99039, 

 99097, 99100 and 92297) gave delayed reactions. Bird 99100 reacted in a similar 

 manner on the previous test, but was permitted to remain in the flock on the 

 strength of the type of reaction. One (99130) of the four reactors was submitted 

 to the laboratory. 



Since reactors continued to appear in two pens and the owner was desirous 

 of eliminating the infection in the shortest possible time, the birds in these two 

 pens were retested at two-week intervals and the whole blood test was supplement- 

 ed with the standard tube test. 



On January 28, 1935, a total of 292 birds was tested. Nine birds were detected 

 as reactors: two (99100 and 99186) reacting to both methods, one (99272) to the 

 whole blood test, and six (91923, 99039, 99077, 99153, 99181 and 99250) to the 

 tube test. Seven of the nine reactors were submitted to the laboratory. Of the 

 other two reactors, one (99077) died shortly after the test and the other (91923) 

 remained on the premises but gave a negative reaction to both methods on the 

 next test. 



On February 11, 1935, the entire flock was tested with both methods and no 

 reactors were detected. Nine delayed reactions were observed with the whole 

 blood test in one pen of birds which had never revealed reactors. On subsequent 

 test these delayed reacting birds were negative. 



On February 25, 1935, no reactors were detected among birds tested on January 

 28. Approximately two weeks later (March 11 and 12) the entire flock was tested 

 with negative results. No further testing was conducted, since the entire flock 

 had passed two consecutive negative tests at least one month apart. 



Table 8. --Summary of tests-Flock B. 

 Date of Test Number of Birds Number of Reactors 



October 2 and 3, 1934 826 29 



October 31 and November 2 1 120 13 



December 10 and 11 976 10 



January 14 and 15, 1935 989 4 



(l W. B. T. 

 January 28 292 9 -j 2 both tests 



[6 tube test 



February 11 and 12 851 



February 25 204 



March 11 795 



Totals 6053 65 



The agglutination studies and necropsy results on the 22 reactors submitted 

 to the laboratory are shown in Table 9. 



