4G 



MASS. KXI'KRIMENT STATION BULLETIN 27.'} 



Table 20. — Rksitlts ok inoculation wni; Rkukefki.d A' filtkai-e of the 

 SECOND California vikus. 



Date 



Chicken No. V 220S 



Chicken No. V 2209 



Chicken No. V 2210 



6/3/30 Inoculated 



6/7/30 Symptoms of 



6/9/30 infectious trachitis 



6/11/30 Dead 



6/14/30 (No lesions) 



6/18/30 



6/21/30 



Inoculated 



Symptoms of 



infectious trachitis 

 Dead 

 (Lesions of 



infectious trachitis) 



Inoculated 



Symptoms of 

 infectious trachitis 



Dead 



( Lesions of 

 infectious trachitis) 



These experiments demonstrate that tlie second California virus did not 

 pass the pores of Berlvefeld W and N filters in sufficient (piantities to 

 iulect the five chiclcens inoculated, aithoujih t!ie birds were all found 

 susceptible to the unfiltered virus later. Sufficient virus passed the pores 

 of the Berkefeld V filters to kill tiie tliree ciiickens inoculated, tlius in- 

 dicating that tlie second California virus was filtrable. Attention slioiild 

 be called to V 220S. as this is the second bird observed in these experi- 

 Uients, ai)]iarently dyinii- of infectious tracliitis, but slu)wing no lesions of 

 disease. 



Demonstration of the Second California Virus in the Tissues of 

 Diseased Chickens 



Since the tissues of the spleens, livers, and kidneys of so many of the 

 birds examined at necropsy showed lesions, such as hemorrhagic inflain- 

 ii'.ation, hyperemia, coagulation necrosis, infiltrations, iiyperj)lasia, and 

 fatty degeneration, it was thought tliat tissues from these organs might 

 yield virus for inoculation purposes. Up to this point tracheal exudate had 

 been depended upon as a source of virus. The supply of tracheal exudate 

 was limited in each case. The need for a greater supi)l\, as well as <i 

 more potent \irus, was keenly felt as these experiments progressed. 



In order to demonstrate the iiresence of \irus in the tissues of chi<'kens 

 (l^ad of infectious trachitis, the following ex])eriments were conducted us- 

 ing the second California virus as the infecting agent: - 



The liver from V 2211 was triturated in a sterile mortar, with a sterile 

 pestle, in enough sterile saline solution to make a suspension, .\fter re- 

 maining in the ice box over night, the liipiid was poured through a sterile 

 gauze. This gau/.e filtrate was run through a Berkefeld \' filter. On 

 June 7, 1930, the clear Berkfeld \'' filtrate was used to swab tlie trache.is 

 oi chickens V 221(5 and V 221S. On .Fune 12, \' 221(i died of infectious 

 trachitis, but V 2218 remained apparcnfl> unatlected. On June 16, V 

 2218 was re-inoculated intratracheally with unfiltered virus from V 2221 

 which had Just died of infectious trachitis. Five days later V 2218 died 

 of the same disease, .•Uiowing that it was not immune. This experiment 

 demonstrated that the virus of infectious trachitis was present in the 



