48 



MASS. EXPERIMENT STATION BULLETIN 273 



mucosa of the intestines. The anterior trachea manifested considerable 

 grayish-white mucus in the midst of which were localized caseated areas. 

 The middle portion of the trachea was slightly hemorrhagic. The bronchi, 

 lungs, larynx, and other internal organs sliowed no lesions. The case was 

 diagnosed as chronic infectious trachitis, culminating in relapse and death. 

 This was the only instance in which a bird contracted infectious trachitis 

 as a result of subcutaneous inoculation of virus. Cross infection was ex- 

 cluded by adequate controls which were kept under the same conditions 

 and remained healthy throughout the experiment. 



The second California virus was demonstrated in the tissues of the 

 spleens, livers, and kidneys of chickens dead of infectious trachitis. TEe 

 virus as measured by dilution and inoculation into healthy chicks by 

 tracheal swabs, was no greater in the tissues of these organs than in the 

 tracheal exudate. 



Tissues of the liver, spleen, kidneys, and trachea may be used along Mith 

 tracheal exudate to provide a larger quantity of virus of fair potency. 



Continuous Passage of the First New Jersey Virus 



Further studies of infectious trachitis viruses were made with two 



sirains received from New Jersey. These were known as the first and 



second New Jersey viruses. Results with the first New Jersey virus are 

 shown in Table 21. 



Table 21. — Transfers of first New Jersey virus. 



Chicken V 2236. — At necropsy the general condition of the carcass was 

 good. The face was pale, and the feathers were ruffled. One small patch 

 »f pseudomembrane was found in the pharynx immediately above the 

 larynx. Desquamative epithelial inflammation of the trachea was evident. 

 The desquamated areas were hemorrhagic. The blood in the heart and 

 large arteries was unclotted, although the bird had been in the ice box 

 several hours previous to necropsy. The liver showed a few areas of 

 fatty degeneration. The kidneys were hemorrhagic. The spleen was con- 

 gested. Except for the unclotted blood, tliis case was typical of infec- 

 tious trachitis as observed hitherto. 



Chicken V 2237. — The general condition was good; the lungs were tough, 



