50 MASS. EXPKKIMENT STATION BULLETIN 273 



Continuous Passage of the Second New Jersey Virus 



The second New Jersey virus was transniitted in series at the same 

 time as the first virus. Tlie results are given in Table 22. 



'i'ABi.E 22. — Transfers or second New Jersey vn<us. 



The second New Jersey virus, like the second California virus, was 

 carried through seven chickens from one to three months of age, for ,x 

 period of one month and nine days without losing in virulence. The 

 hemorrhagic sequala associated with the lesions of the first New Jersey 

 virus were noticed in the chickens dead of the second virus. 



The Filtrability of the First New Jersey Virus 



The trachea was removed from chicken \' 2238 as aseptically as pos- 

 sible, ground up with sterile saline solution in a mortar, and placed in 

 the ice box over night. The next day, July 12, 1930, the solution was run 

 through a Berkefeld V filter, after the coarser particles had been re- 

 moved by filtration through sterile gauze; and two cliickens, V 2246 and 

 Y 224-7, were inoculated intratraclieally with the filtrate. No reaction oc- 

 curred in seven days. On .July Iff these chickens were inoculated with 

 unfiltered exudate from the trachea of \' 2245. No reactions occurred. 

 It was concluded that these chickens were immune. 



Chickens V 2242 and V 2243 were inoculated intratracheally with Berke- 

 feld N filtrate from the trachea of chicken \' 2237. No reactions took 

 ]ilace for eleven days. On July 19, these chickens were inoculated with 

 virus from A' 2245. Chicken V 2242 died of infectious trachitis in fi\e 

 days and V 2243 died of the same disease in nine days. The symjjtoms 

 and pathology were typical of the disease under investigation. 



On July Ifi chickens V 2252 and V 2253 were inoculated intratraclieally 

 with virus from \' 2244 and \' 2245 after it had i)assed through a Seit/, 

 filter. Seven days later both chickens died of infectious trai-hitis. 



These exi)eriments demonstrated that the virus of the first New Jersey 

 strain of infectious trachitis M'as filtrable, as it readily passed the Seitz 

 filter, but it did not pass the Berkefeld N fdters in sufficient quantities to 

 cause infectious trachitis in chickens inoculated intratraclieally with the 

 filtrate. 



