METHODS OF CULTURE 149 



Laurent's Medium: 



Ammonium sulfate 4. 71 grams.. 



Potassium phosphate , 0. 75 



Magnesium sulfate 0.1 



Distilled water 1000.00 



To this medium any sugar may be added. It was used by Laurent 

 in his investigations on the hydrocarbon nutrition studies on yeasts. 



Haydruck's Medium: 



Water 2000 grams. 



Saccharose 100 



Asparagin 2.5 



Potassium acid phosphate 50. 00 



Magnesium sulfate 17. 00 



Cohn's Solution: 



Distilled water 200 grams. 



Ammonium tartrate 2 



Potassium phosphate 2 



Magnesium sulfate 1 



Calcium phosphate (dibasic) 0.1 



Sugar 20 



Noegeli's Medium (No. 3). 



Distilled water 100 grams. 



Glucose 3 



Ammonium tartrat ^ 0. 04 



Magnesium sulfate 0. 04 



Calcium chloride 0.02 " 



Raulin's Medium: 



Distilled water 1500.00 grams. 



Candied sugar 70. 00 ' 



Ammonium nitrate 4. 00 



Tartaric acid '. 4. 00 



Potassium carbonate 0. 60 



Magnesium carbonate 0. 60 



Ammonium sulfate 0.25 " 



Ferric sulfate 0.07 " 



Zinc sulfate 0.07 " 



Potassium sulfate 0. 07 " 



Potassium silicate 0. 07 " 



This liquid, composed by Raulin in the course of investigations 

 on the nutrition of Sterigmatocystis nigra, makes up a medium which 

 is well adapted to the develpoment of fungi. It serves also for the 

 growth of many fungi and molds. For the yeasts, however, it does 

 not lend itself as well since they seem unable to grow in it. For a 

 certain few special yeasts it will work. 



