258 



FAMILY OF SACCHAROMYCETACEAE 



Germination of the spores is always similar to that in S. Lud- 

 wigii. The spores fuse two by two by a copulation canal and later 

 germinate by ordinary budding. It often happens, in unfavorable 

 solid media, that the spores after having united two by two, change 

 immediately into normal ascs 



Fig. 121-D. Germination 

 and Copulation of Spores 

 in Yeast from Pulque No. 2. 



Fig. 121-E. Ascs from Yeast from Pulque 

 No. 2 on Beer Wort Agar at 30 C. 



This yeast inverts and ferments saccharose. It is curious and 

 aberrant having certain analogies, in the formation of its mycelium 

 and other characteristics, to Saccharomyces Ludwigii. 



ZYGOSACCHAROMYCES SO JA. Takahashi and M. Yukawa l 

 Syn.: SACCHAROMYCES SOJA. Saito 2 



This yeast was isolated during the early stages of ripening of 

 "Shoju Moromi," and seems to be an important species for "Shoju " 

 manufacture. Excepting the fermentability of galactose, Saccharo- 

 myces soja seems to be similar to this yeast; moreover, there is not a 

 great difference between Torula "Shoju " and this yeast. According 

 to Saito's illustration it is questionable that he, who gave the name 

 of Zygosaccharomyces japonicus to this "Shoju " film yeast, comprised 

 his "Shoju " yeast into the genus of Saccharomyces. Jorgensen 3 

 also has the same inference about this question. 



In "Koji " extract or wort after 5 days at 20 C. the young cells 

 are commonly spherical or oval, 3.5-8 ju in diameter. The contents 

 are homogeneous and sometimes exhibit vacuoles, and are rich in 

 glycogen. The cells of old cultures in "Koji" extract or wort after 



1 Takahashi, and Yukawa M. Original communications, Eighth Internatl. 

 Congress of Applied Chemistry, v. XIV, 1912, p. 166. 



2 Saito, K. Cent. f. Bak. II, Ab. XVII, 1906. 



3 Jorgensen. Die Microorganismen d. Garungsindustrie. IV. Aufl. Jorgen- 

 sen, 370. 



