SACCHAROMYCES ETIENNE 263 



A feeble ring is formed at 25-30 on beer wort after about 12 days. 

 This ring is made up of spherical or oval cells united in groups. The 

 mycelial formation has not been observed. 



Spores are formed quickly on slices of carrot, Gorodkowa's medium 

 and the plaster block. The temperature. limits on plaster block are 

 maximum 39-40 and minimum 8-10. The optimum is situated at 

 about 25-30. At this temperature the spores appear in about 12 

 hours. The spores are to a number of from 1 to 4 per asc. They 

 are spherical and have a diameter of from 2.5 to 3.5 microns. 



Germination is generally preceded by sexual processes analogous 

 to those which have been described for Johannesburg II yeast. At 

 the beginning of germination the spores enlarge; the wall of the asc 

 disappears, but may persist during the first stages of germination. 

 About one-fourth of the spores germinate only by ordinary budding 

 without preliminary copulation. The others unite two by two by 

 means of a copulation canal. 



On wort agar in streaks S. Chevalieri produces at the end of three 

 days a train of grayish white growth with a slightly indented border; 

 at the end of 15 days to a month the colony is white with a damp 

 appearance; its center is thick and border slightly undulated. On 

 wort gelatin in stab cultures the yeast develops a colony which is 

 funnel shaped. The surface has a damp appearance; it is thick at 

 the center and thin at the edges. The giant colony on wort agar 

 at the end of 15 days at 25 is well developed, spherical, slightly 

 moist, and has a grayish white color. It is made up of a central granu- 

 lar portion and a thin transparent peripheral part. 



S. Chevalieri has the characteristics of a top yeast. It causes a 

 rather active fermentation on beer wort. It ferments saccharose, 

 dextrose, levulose and d-mannose quite actively, but does not seem 

 to have any action on galactose, maltose or lactose. 



SACCHAROMYCES ETIENNE. Potron l 



This yeast was isolated from sputum from a disease in which it 

 was the causal agent. The infection began with a gastro-enteritis 

 which later turned into a pleuro-pulmonary trouble which had some 

 of the appearances of tuberculosis. The sickness yielded to treat- 

 ment with iodine. The yeast develops on carrot and potato. It has 

 cells which vary from spherical to ellipsoidal in shape (39 fj, long 

 and 4-5 /z wide). Curled cells are more numerous in scums and old 

 cultures. The ascospores appear on carrot after 30 hours. The 



1 Potron. Presence d'une levure au course d'une infection pleuropulmonaire 

 grave. Soc. de Med. de Nancy, 1914. 



