274 FAMILY OF SACCHAROMYCETACEAE 



circumstances the cells may be half-moon shaped or elongated. The 

 buds, in the form of a lemon, develop especially in the first phases 

 of the culture and later may be replaced by buds oval in shape. The 

 cells never contain glycogen. 



Engel has reported the observation in this yeast of ascogenous 

 fructification related to that of Protomyces and has created the genus 

 Carpozyma for it. On the contrary, Hansen has never noticed ascs 

 or other forms of fructification in this yeast and regards it as be- 

 longing to the Torula. However, Beijerinck (1894) 

 reported the presence of ascs with many ascospores. 



OV/ Klocker, however, has been unable to confirm the 

 J^\^ presence of ascs and thinks that Beijerinck has taken 

 /^\ for ascospores the fat globules which are commonly 



present in the cells of this yeast. 



Lindner has demonstrated the formation of ascs 

 ^n Hanser^i m a Saccharomyces apiculatus isolated from the flowers 

 ajnculata (after of Robinia pseudoacacia. The ascs never contain but 

 a single ascospore. (Fig. 127.) He was not successful 

 in observing the germination of these ascospores. Rohling has been 

 able to follow the germination of one of these ascospores in a decoc- 

 tion of horse manure to which 5 per cent of glucose was added. It is 

 then probable that special conditions are necessary for their germina- 

 tion. 



In the light of these discoveries, Lindner has created for this species 

 a new genus Hansenia. But according to Klocker, 1 Saccharomyces 

 apiculatus in which Lindner has described ascospores, may not be 

 identified with the Saccharomyces apiculatus of Hansen but may only 

 be a species related to this yeast, for in the true S. apiculatus, under 

 no circumstances may the presence of ascs be observed. Indeed, all 

 of the efforts put forth by Hansen and Klocker to demonstrate spores 

 in this species have been in vain. 



Zikes, 2 on the other hand, has tried to make Saccharomyces api- 

 culatus sporulate by various procedures but has had little success. 

 He admits that Hansenia apiculata is different from S. apiculatus 

 and proposed to designate the Saccharomyces apiculatus of Hansen 

 under the name of Hanseniaspora mucroniata (Lindner). The genus 

 Hanseniaspora would be part of the family of Saccharomycetes while 

 the genus Hansenia would be placed among the Non-Saccharomycetes. 

 It may be regarded as an asporogenic variety of Hansenia or as a spe- 

 cial form not having all of the characteristics of the latter. 



1 Klocker, A. Invertin und Sporenbildung bei Saccharomyceten apiculatus- 

 Formen. Cent. Bakt. 21, 1910. 



2 Zikes, H. Zur Nomenclaturfrage der Apiculatushefe. Cent. Bakt. 30, 1911. 



