PICHIA HYALOSPORA 



277 



PICHIA MEMBRANAEFACIENS II AND III. Pichi 

 Syn.: SACCHAROMYCES MEMBRANAEFACIENS n AND m. Pichi 



Pichi 1 has described two species of yeast which very much re- 

 semble P. membranaefaciens and which he has called Saccharomyces 

 membranaefaciens II and Saccharomyces membranaefaciens III. These 

 two species have somewhat the same 

 physiological and morphological charac- 

 teristics of Saccharomyces membranae- 

 faciens of Hansen. They seem to 

 differ only in the shape of their ascs. 

 P. membranaefaciens II was found 

 on the leaves of Evonymus europaeus. 

 The cells are usually 5 to 7 /z long and 

 3.5 IJL wide and even 10-19 ju long and 

 3-4 to 5 JJL wide. The ascospores are 

 round or a little flattened (2.5-3 ju). 

 The ascs enclose 3-4 ascospores and 

 form in a milky white, folded scum. 

 They are oval (6-8 // long by 3-5 JJL Fig. 130. Pichia membranaefaciens 

 wide). (after Kayser) ' 



P. membranaefaciens III was isolated from wine. The cells are 

 5 to 7 jtt long and 3-4 to 6 JJL wide. The ascospores (2.5 X 3.5 JJL) are to 



the number of 2-4 in a spherical or 

 oval asc. The scum develops on beer 

 wort at 22-25 C. and is folded with 

 a large number of ascs. 



PICHIA HYALOSPORA. Lindner 



Syn.: SACCHAROMYCES HYALOSPORUS. 

 Lindner 



This yeast, discovered by Lindner, 

 forms on beer wort a delicate scum. 

 The ascospores are rounded, each being 

 provided with a brilliant granule in 

 the center probably of the nature of 



'& JLUJ.. *^. e.yw/iye>fyi/ u,. v^tllLUiC 



on Saccharose Gelatin. Vegeta- a fat globule (Fig. 131). The giant 

 live Cells and Ascs (after Lindner). co l onies are a dull gray with a maceo us 



appearance. This yeast does not induce a fermentation. 



1 Pichi, P. Ricierche morph. e fisilogische sopra due nuove specie di Sacch. 

 prossime al S. membranaefacfens. Ann. della R. Scuola di viticoltura e di Enologia 

 in Conegliano, 1, 1892. 



Fig. 131. S. hyalospora. Culture 



