PICHIA CALLIPHORA 



281 



a large refractive globule in the center. Each asc contains about 2 

 ascospores. Ascospores are formed with difficulty on plates. The 

 temperature limits for sporulation on plates are 29-33 C. and 10 C. 

 It ferments dextrose and saccharose only. In beer wort it sets up a 

 feeble fermentation with a fruity odor. Gelatin is not liquefied. 



PICHIA ALCOHOLOPHILA. Klocker 



This species was found in Denmark. On must after a few days it 

 forms a scum at 25 C. more or less developed. On must with 10 per 

 cent of alcohol added, it forms a well-developed 

 scum. It is folded and adheres to the walls 

 of the flask. There is abundant sediment al 

 growth. The cells in the scum are spherical; 

 those of the sediment are oval or sausage 

 shaped. The temperature limits for the forma- 

 tion of the scum are 33-35 C. and 3.4 to 8.4 

 C. The ascospores are to the number of four, 

 sometimes two per asc. They are spherical 

 or hemispherical. They are formed abun- 

 dantly in the scums. The limits of tempera- 

 ture for the formation of spores on plates are 29-33 C. and 0.5-4 C. 

 Gelatin is partially liquefied. Dextrose is feebly feimented. 



Fi - 7 13 ?7 7 A -r: Pic ^ a ^ lco " 

 holophila (after Klocker). 



Cells from a Young Scum 

 on Beer Wort to which 

 Alcohol was Added. 



PICHIA POLYMORPHA. Klocker 



This species was also found in the soil of 

 Denmark. A white well-developed scum is formed 

 on must in 24 hours at 25 C. It adheres to the 

 sides of the flask. At first the cells are elongated 

 or egg-shaped (13 ju long). They bud laterally. 

 Fig. 132-B. Pichia Finally, they become spherical or oval. The tem- 



polymorpha (after perature limits for scum formation are 39 and 0.5 

 Klocker). Cells ^ r , . 



from a Young ^ The ascospores are spherir 



Scumon Beer Wort cal and formed with difficulty 

 on plates. Gelatin is liquefied. 

 Dextrose and saccharose are fermented and maltose 

 feebly. 



PICHIA CALLIPHORA. Klocker 



Fig. 132-C. Pichia 

 calliphora (after 

 Klocker). Cells from 

 a Young Scum on 

 Beer Wort at 25 C. 



This species was found on the fly Calliphora 

 arthrocephala at Carlsberg. On beer wort, a white 

 scum is formed along with a feeble ring. The cells are sausage shaped 

 and almost 13 /it long. The temperature limits for scum formation are 



